FastQCFastQC Report
Tue 27 Jun 2023
ERR618771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR618771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161912593
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCA45328932.799592617233917No Hit
AAATGCCCTGCTTCCAGGAAAAGCC30309831.8719871900266583No Hit
AGCGAAGCTCTTGATCGAAGCCCCG27683081.7097545958021931No Hit
GCGGGAATAGCTCAGTTGGTAGAGC17936091.1077637426262452No Hit
GGTTAAGCGACTAAGCGTACACGGT16493891.0186909921206684No Hit
ACGGACGTGGGGGTTCAAGTCCCCC15283080.9439092856724245No Hit
AGAATGCCACGGTGAATACGTTCCC14096540.8706265361336039No Hit
GAGGCTGAGGCGTGATGACGAGGCA11869140.7330584842156163No Hit
AAGTCATCATGGCCCTTACGACCAG11620170.7176816691460187No Hit
ATCAGGTAACATCAAATCGTACCCC11595020.7161283619242637No Hit
AGACCGCCAGCTAAGGTCCCAAAGT9898530.6113502240063563No Hit
CCTGCGGTTGGATCACCTCCTTAAA9637100.5952038579235155No Hit
ACCGCCAGCTAAGGTCCCAAAGTCA9620570.5941829367157377No Hit
AAGCGTGTAGGCTGGTTTTCCAGGC8252510.5096892000241142No Hit
GCGAAGGTGGCGGAATTGGTAGACG7632460.4713938464316979No Hit
GGAGCGGTAGTTCAGTCGGTTAGAA7443760.4597394101396425No Hit
ATGAAAGCTACTAAACTGGTACTGG7045790.435160099004776No Hit
ACACGGCGGGTGCTAACGTCCGTCG6364750.3930978981974552No Hit
AGGCGTGTGACTGCGTACCTTTTGT5993980.37019850580738956No Hit
AGGTTAATGAGGCGAACCGGGGGAA5747650.35498474167478744No Hit
GGGGCTGATTCTGGATTCGACGGGA5648610.34886786106871875No Hit
CCTGCGGTTGGATCACCTCCTAAAA5350180.33043631139920043No Hit
ATGCGTTGTTGGGTAGGGGAGCGTT5294330.32698691941768854No Hit
CCTGCGGTTGGATCACCTCCAAAAA5233520.32323118931212474No Hit
AATGCCCTGCTTCCAGGAAAAGCCT4743280.29295312440583293No Hit
ATGCAAGTCGAACGGTAACAGGAAG4683410.2892554503157145No Hit
ACATGCAAGTCGAACGGTAACAGGA4294420.26523075941350654No Hit
AGGTAACATCAAATCGTACCCCAAA4236560.26165722637769134No Hit
AAACGATGTCGACTTGGAGGTTGTG4155160.2566298224870007No Hit
AAATTGAAGAGTTTGATCATGGCTC3989390.24639158240149978No Hit
CGGACGTGGGGGTTCAAGTCCCCCC3976770.24561214951328708No Hit
GCATCCGTAGCTCAGCTGGATAGAG3897300.2407039457394151No Hit
AGGCTGAGGCGTGATGACGAGGCAC3797690.23455186095376782No Hit
GGGGCTATAGCTCAGCTGGGAGAGC3789970.23407506048649346No Hit
TCAAACCGGGAGATAGCTGGTTCTC3738610.23090297862131082No Hit
AGTAGGTAGCTTAACCTTCGGGAGG3689320.22785874351354496No Hit
ATGCAAGTCGAACGGTAACAGGAAA3654800.2257267289888934No Hit
AGGCTTGTAGCTCAGGTGGTTAGAG3626250.2239634319240382No Hit
AACAAATGCCCTGCTTCCAGGAAAA3579170.22105569021428742No Hit
AAATGAATTGACGGGGGCCCGCACA3515240.21710726354681997No Hit
TAGGCTGAGGCGTGATGACGAGGCA3432370.2119890699298479No Hit
CGGAGGTTCGAATCCTCCCGGATGC3408050.2104870249344966No Hit
ATCTAAGTACCCCGAGGAAAAGAAA3387070.20919126407913188No Hit
GAATGCCCTGCTTCCAGGAAAAGCC3312990.20461595596829213No Hit
AGGCACGCTGATATGTAGGTGAAGC3261980.20146549070460507No Hit
AAAATGGTGCCGTAACTTCGGGAGA3125380.19302883994946582No Hit
ACTTGCCCCGAGATGAGTTCTCCCT3042040.18788161832477107No Hit
AGCGAAATTCCTTGTCGGGTAAGTT3028670.18705586414763922No Hit
TAGGCGTGTGACTGCGTACCTTTTG2937440.1814213425635151No Hit
TGAGGCGTGGCTTCCGGAGCTAACG2905640.17945731991334363No Hit
TAGCGAAGCTCTTGATCGAAGCCCC2874100.17750935530999742No Hit
TGATCGAAGCCCCGGTAAACGGCGG2730540.1686428429936886No Hit
TGCTGTTTCGCTGACGAGTGGCGGA2726780.16841061893190729No Hit
AGTGATCCGGTGGTTCTGAATGGAA2686580.16592779784584144No Hit
AAGCAGTGGGAGCACGCTTAGGCGT2660370.16430902320241392No Hit
TCCCCATGCGAGAGTAGGGAACTGC2631680.16253707949696045No Hit
AACCGAGATTCCCCCAGTAGCGGCG2608890.1611295299310042No Hit
ACGGTCCAGACTCCTACGGGAGGCA2607660.1610535630171768No Hit
GAGTCATCATGGCCCTTACGACCAG2556060.15786665833954003No Hit
GCGAAGCTCTTGATCGAAGCCCCGG2550650.15753252744213664No Hit
GATGCCCTGCTTCCAGGAAAAGCCT2534100.15651037099998763No Hit
TACGGACGTGGGGGTTCAAGTCCCC2433920.1503230820347618No Hit
ATTCATCTCCGGGGGTAGAGCACTG2422890.14964185027905766No Hit
AAGTAGGTAGCTTAACCTTCGGGAG2417260.14929413180357132No Hit
AGAAGTGCGAATGCTGACATAAGTA2374020.14662355509308655No Hit
TAGGTTAATGAGGCGAACCGGGGGA2368640.14629127704723993No Hit
TCGCGCTGTGCGGGTTCAAGTCCCG2325350.14361761225082723No Hit
GTGTAGGCTGGTTTTCCAGGCAAAT2315480.14300802408865135No Hit
TCCTCTGTAGTTCAGTCGGTAGAAC2278460.14072160526760263No Hit
GGAATGCCACGGTGAATACGTTCCC2271510.14029236132361858No Hit
ACTGGAACTGAGACACGGTCCAGAC2246990.13877796398455555No Hit
ATAATGGGTCAGCGACTTATATTCT2243300.1385500632430734No Hit
ACTGCGAATACCGGAGAATGTTATC2200250.1358912212591148No Hit
ATGCCCTGCTTCCAGGAAAAGCCTC2175760.1343786767716085No Hit
ATAAAGTGCGTCGTAGTCCGGATTG2130630.1315913704130475No Hit
AGGGCTTACGGGTTCAAGTCCCGTC2102180.12983424952004813No Hit
ATAGTGATCCGGTGGTTCTGAATGG2060560.1272637267936287No Hit
AACTGTTTATTAAAAACACAGCACT1979740.12227214469970227No Hit
AAACCGGGAGATAGCTGGTTCTCCC1975780.1220275682942092No Hit
AAGGCGCTTGAGAGAACTCGGGTGA1925310.11891045435854393No Hit
GAGCGTGTAGGCTGGTTTTCCAGGC1882700.11627878753075124No Hit
AAAGTGCGTCGTAGTCCGGATTGGA1872750.11566425843109066No Hit
TCGAAGCCCCGGTAAACGGCGGCCG1856730.11467483569977785No Hit
ATGGCCCTTACGACCAGGGCTACAC1841880.11375767417917887No Hit
ACCCAGACCGCCAGCTAAGGTCCCA1822650.11256999633129217No Hit
ACGATGTCGACTTGGAGGTTGTGCC1815660.11213828191856579No Hit
AAGCTCTTGATCGAAGCCCCGGTAA1814610.112073432114079No Hit
TGAAAGCTACTAAACTGGTACTGGG1812990.11197337813001365No Hit
ACCGGAGTGGACGCATCACTGGTGT1812410.11193755633324952No Hit
AAGTCGAACGGTAACAGGAAGAAGC1809750.11177327016188296No Hit
AACTTGCCCCGAGATGAGTTCTCCC1765140.10901807989697256No Hit
AATGGGTCAGCGACTTATATTCTGT1764240.10896249435026958No Hit
GCTCAGTTGGTAGAGCAGCGCAAAA1753420.1082942325554628No Hit
AGTGGGGAATATTGCACAATGGGCG1734970.10715472884805198No Hit
AAACTGCGAATACCGGAGAATGTTA1708650.10552916041558298No Hit
CGCAGGTTCGAATCCTGCACGACCC1686430.1041568150292053No Hit
AGGTAGCGCCTCGTGAATTCATCTC1684830.10405799627951112No Hit
TTAGGTTAATGAGGCGAACCGGGGG1683490.10397523557664227No Hit
GAGATTCCCCCAGTAGCGGCGAGCG1656960.10233669718327591No Hit
AGCTCTTGATCGAAGCCCCGGTAAA1626590.10046099379064358No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)