FastQCFastQC Report
Wed 27 Sept 2023
ERR653341.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653341.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109005852
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC59922835.497212204717229No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG28962512.656968361661904No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG18977991.741006528713706No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT14123211.2956377791533615No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC10103060.9268364784672295No Hit
CCGTGGCGCAATGAAGGTGAAGGGC7650410.701834796906133No Hit
TTTTCACTGACCCGGTGAGGCGGGG6682490.6130395641511064No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC5621670.5157218531716994No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCG5490840.5037197452481725No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC5116990.46942342141410903No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG4377060.40154357951351094No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG4323680.3966465947167681No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC4028780.3695930013005173No Hit
CCGTGGCGCAATGAAGGTGAAGGGCC3841270.35239117253998437No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGC3623620.3324243546117139No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT3408040.31264743474506307No Hit
TCGACTGCATAATTTGTGGTAGTGGGG3223350.2957043076916641No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC3090180.2834875323941324No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2994540.2747136915181398No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC2947720.27041850927416267No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT2538190.23284896667749544No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC2392260.21946161202427922No Hit
CGCCGTCGGGCCCGGGGGGCCGTCGTCACGCG2387740.21904695538731261No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2185460.20049015350111662No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC2183420.20030300758531752No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCG2161810.19832054521256348No Hit
ACAACCCGCAGTTTTATCCGGTAAAGC2147580.19701511071167077No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC1995370.18305164020001422No Hit
CGCGGGGGCCGGGCCGCCCCTCCCACGGCG1985560.18215168851668623No Hit
TTTTTCACTGACCCGGTGAGGCGGGG1954440.17929679591880993No Hit
TTTCACTGACCCGGTGAGGCGGGG1787350.16396826108014825No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1765460.16196011201306879No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAT1752960.1608133845878293No Hit
CGACTGCATAATTTGTGGTAGTGGGG1674400.15360643206568395No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC1661500.1524230093628368No Hit
CGCGGGGGCCGGGCCGCCCCTCCCACGGCGC1631590.14967911997972366No Hit
TACCCGTCGCCGCGCTCTCCCCCCGGCC1625040.14907823480889815No Hit
CTCGGTTGGCCCCGGATAGCCGGCTCCCCG1431240.13129937280798465No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGC1363600.12509420136452856No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1342490.12315760808878408No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1321780.12125771009064724No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGC1265570.11610110620483018No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1248720.1145553176356073No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC1211150.1111087136863074No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC1120690.10281007665533407No Hit
GACAACCCGCAGTTTTATCCGGTAAAGC1116760.10244954555283876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)