FastQCFastQC Report
Wed 27 Sept 2023
ERR653342.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653342.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102415831
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC42124804.1131141141646355No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG28819272.813946800861285No Hit
TTTTCACTGACCCGGTGAGGCGGGG9198800.8981814539980639No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT8871420.8662156927672637No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG8575180.8372904770943079No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC5958140.5817596695573363No Hit
CCGTGGCGCAATGAAGGTGAAGGGC4885760.47705124806339755No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG4669860.45597052276029476No Hit
CCGTGGCGCAATGAAGGTGAAGGGCC3974890.3881128494675789No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC3486530.34042881515065776No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC3124170.3050475663279049No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGC2840020.2773028322154609No Hit
TTTTTCACTGACCCGGTGAGGCGGGG2636500.2574309044077375No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT2548380.24882676585419689No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC2531780.24720592268591754No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG2482510.24239514299307885No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2425740.236852054639873No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCG2289370.22353673037130364No Hit
TCGACTGCATAATTTGTGGTAGTGGGG2260690.2207363820540596No Hit
TTTCACTGACCCGGTGAGGCGGGG2131510.20812309768789553No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC2094720.2045308796059078No Hit
ATCGTATAGTGGTTAGTACTCTGC1936280.18906061505276464No Hit
CTCGGTTGGCCCCGGATAGCCGGCTCCCCG1885930.18414438291283308No Hit
ACAACCCGCAGTTTTATCCGGTAAAGC1808280.17656254724916504No Hit
TACCCGTCGCCGCGCTCTCCCCCCGGCC1780850.17388425037531552No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC1755050.17136510858365245No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1734460.1693546772080578No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1659670.16205209524687644No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC1637090.1598473579733977No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGAGG1608290.15703529271758776No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC1540610.15042693936643448No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1435100.1401248211323892No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG1420660.1387148828582956No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1244130.12147828981634685No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC1161850.11344437560634546No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1160850.11334673445162985No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAT1145350.1118332965535377No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGC1131580.11048877785310358No Hit
CGCGGGGGCCGGGCCGCCCCTCCCACGGCGC1069320.10440963956050897No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC1028490.10042295121347011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)