FastQCFastQC Report
Wed 27 Sept 2023
ERR653343.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653343.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84733114
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTCTGGCGCCAAGCGCCCGTCCG49502425.842157530053717No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT12575061.4840785858525158No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC11208071.3227496867399444No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC7390730.872236325458309No Hit
CTCGGTTGGCCCCGGATAGCCGGCTCCCCG5907100.6971418517676572No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGC5702980.6730520962560163No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG5400040.6372998400601683No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC5020350.5924897319364422No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG4072200.48059133056292486No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG3959710.4673155290858306No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC3892660.4594024480204988No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCG3431530.40498098535597316No Hit
CCGTGGCGCAATGAAGGTGAAGGGCC3316340.391386536319201No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG3154900.37233377260276307No Hit
TCGACTGCATAATTTGTGGTAGTGGGG2879560.3398388025725102No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2625360.3098387249169197No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2574880.30388119572709205No Hit
TTTTCACTGACCCGGTGAGGCGGGG2531120.2987167449080179No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCG2132090.2516241761160814No Hit
CCTCGGTTGGCCCCGGATAGCCGGCTCCCCG2082370.2457563403134222No Hit
CGCGGGGGCCGGGCCGCCCCTCCCACGGCGC2034130.24006317058051235No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG1999370.23596087829369758No Hit
TCGGTTGGCCCCGGATAGCCGGCTCCCCG1993460.2352633941908473No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC1961350.23147384858297546No Hit
CCCGGGGAGCCCGGCGGGTCGCCGGCGCGG1872270.2209608394659023No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1772320.2091649788770893No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGG1744010.2058239002050603No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1741180.20548991035547215No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC1730550.20423538311125916No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT1688920.19932230981148644No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1680910.19837698871777568No Hit
CCGTGGCGCAATGAAGGTGAAGGGC1622680.191504823014058No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCATTC1584480.18699655013269073No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG1518340.17919086509673185No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1480380.1747109164428915No Hit
AGCGAAGCCGAGCGCACGGGGTCGGCGGCGA1444460.17047172372302993No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG1442810.1702769946587824No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1435610.16942726783297496No Hit
TGGGGGGGATGCGTGCATTTATCAGA1425380.1682199476346402No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1320840.15588238619437497No Hit
CGCGGGGGCCGGGCCGCCCCTCCCACGGCG1307570.15431629244736597No Hit
CGCCGTCGGGCCCGGGGGGCCGTCGTCACGCG1298560.15325295373895972No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1289860.15222620049110905No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGG1289470.1521801736213778No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT1246290.1470841730188271No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1228320.14496339648274936No Hit
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG1213840.14325450142195884No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1188930.14031468263989447No Hit
TCGACAACCCGCAGTTTTATCCGGTAAAGC1177730.13899288535530513No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATT1175210.13869548096627252No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC993700.11727410372289633No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG986720.1164503407723219No Hit
GCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC978760.1155109205593459No Hit
TTTCACTGACCCGGTGAGGCGGGG918090.10835079187577125No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG910350.10743733553802827No Hit
AACGATGCCGACTGGCGATGCGGCGGCG896270.10577564752311593No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG878710.10370325820906336No Hit
ATCGTATAGTGGTTAGTACTCTGC848240.10010726148929212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)