FastQCFastQC Report
Wed 27 Sept 2023
ERR653344.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653344.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46907431
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTCTGGCGCCAAGCGCCCGTCCG10072572.147329279235096No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5562781.1859059175506754No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC4746201.011822625715742No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC2698270.5752329518962571No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC1946230.4149086740648833No Hit
CTCGGTTGGCCCCGGATAGCCGGCTCCCCG1786070.3807648302035556No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGC1690510.3603927914960851No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG1643170.3503005739111997No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1617970.3449282907861656No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1577920.3363901979624508No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1487760.3171693627817733No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1322670.28197451273765134No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC1271500.271065793392096No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGA1107120.23602230529316345No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1039290.22156190988161345No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG878380.18725817664156452No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC859890.18331637049148994No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT840520.17918696080371574No Hit
TTTTCACTGACCCGGTGAGGCGGGG837920.17863267762414872No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT817020.17417709360378317No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT789860.16838696623569088No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC778940.16605897688150945No Hit
TTTGGTGACTCTAGATAACCTCGGGCCG713050.15201216199625173No Hit
CCTCGGTTGGCCCCGGATAGCCGGCTCCCCG704150.1501148080354262No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC697860.14877386911255064No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATC672030.1432672789093907No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCG638980.13622148695374087No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC618870.13193431974562836No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG581260.12391640036735331No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAT578180.1232597879854047No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG570680.12166089419819218No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGC555320.11838635972198094No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG548910.11701983849850997No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG546640.1165359066455803No Hit
TTGGTGACTCTAGATAACCTCGGGCCGA540210.11516512170534346No Hit
TCGACAACCCGCAGTTTTATCCGGTAAAGC524790.1118777960788345No Hit
TCGGTTGGCCCCGGATAGCCGGCTCCCCG519470.11074364741910508No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA516760.11016591379732564No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCATTC512520.10926200584295481No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG492130.10491514660011972No Hit
CGCGGGGGCCGGGCCGCCCCTCCCACGGCGC483280.10302845193120895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)