FastQCFastQC Report
Wed 27 Sept 2023
ERR653345.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653345.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47911686
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC6468561.3501006831610978No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT6301041.315136353164445No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG2908380.6070293581403083No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG2476930.5169782587070719No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC2256860.471045832117033No Hit
CTCGGTTGGCCCCGGATAGCCGGCTCCCCG2252460.47012747578951825No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC2186430.45634586935638205No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC1682090.35108136248847516No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1455540.3037964474888235No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1365030.2849054403971507No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1360510.2839620379879765No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG1256230.2621969930258768No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC1195290.24947775788979748No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT978950.20432384700467437No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAT968260.2020926585635079No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG948910.19805397789591458No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT847330.17685246977115354No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT844170.17619292295412023No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC833380.17394086277823745No Hit
CCTCGGTTGGCCCCGGATAGCCGGCTCCCCG817370.17059929805016671No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC762340.15911358243581744No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG758420.15829541043494066No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGA643420.1342929155112596No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG639130.13339751809193273No Hit
CGACTGCATAATTTGTGGTAGTGGGGG635950.13273379692795617No Hit
GACAACCCGCAGTTTTATCCGGTAAAGC572120.11941136865857736No Hit
AGCCAACAAGTACCGTAAGGGAAAGTTG557610.11638287995125031No Hit
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG555900.11602597328760253No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGCG547250.1142205682346474No Hit
CGCCGTCGGGCCCGGGGGGCCGTCGTCACGCG545210.113794784846436No Hit
TTTGGTGACTCTAGATAACCTCGGGCCG541620.11304548957012284No Hit
GTCTAGCGGTTAGGATTCCTGG513150.10710330669640807No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG503860.10516432254126895No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGC500660.10449642703034914No Hit
TTTTCACTGACCCGGTGAGGCGGGG498600.10406646929519449No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCG483690.10095449364900247No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGC481430.10048279244441534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)