FastQCFastQC Report
Wed 27 Sept 2023
ERR653351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77021967
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC23182773.0098906718391127No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG7775361.0094990173387808No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG7489140.9723381902204601No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4029240.5231286809385172No Hit
TTTTCACTGACCCGGTGAGGCGGGG2819050.3660059733348539No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC2754940.35768237391288643No Hit
ACAACCCGCAGTTTTATCCGGTAAAGC2607760.33857353967602516No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC2422610.3145349429987941No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT2339010.3036808966460179No Hit
TCGACTGCATAATTTGTGGTAGTGGGG2098740.2724859000290138No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC2088430.27114732086756493No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1828430.23739071737806958No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGC1799680.23365801603067343No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1615250.20971289917849023No Hit
CCGTGGCGCAATGAAGGTGAAGGGC1561540.2027395638961025No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC1267930.16461927024013812No Hit
CCGTGGCGCAATGAAGGTGAAGGGCC1139090.1478915748801897No Hit
TTTTTCACTGACCCGGTGAGGCGGGG1120030.145416956178229No Hit
CGACTGCATAATTTGTGGTAGTGGGG1008690.1309613398993043No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC994140.129072268434796No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC980110.12725071017726672No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC829480.10769395177871789No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG823050.1068591250078046No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT788620.10238897170725333No Hit

[OK]Adapter Content

Adapter graph