FastQCFastQC Report
Wed 27 Sept 2023
ERR653352.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653352.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71182723
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC26337893.7000396851915878No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG11863881.6666797082207714No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG7501561.0538456080136187No Hit
TTTTCACTGACCCGGTGAGGCGGGG6550320.9202120576365139No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT6274170.8814175316108657No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC5313740.7464929376191467No Hit
CCGTGGCGCAATGAAGGTGAAGGGC4489590.6307134387089969No Hit
TTTCACTGACCCGGTGAGGCGGGG3590070.5043456963566848No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC2984070.419212678896816No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2863700.4023026767323863No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGC2398760.3369862656139187No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT2222160.3121768747171979No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG2037100.2861789931806908No Hit
CCGTGGCGCAATGAAGGTGAAGGGCC1914180.2689107580219992No Hit
ACAACCCGCAGTTTTATCCGGTAAAGC1851320.26007996350462737No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1784570.2507026880665973No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC1775280.24939759609926693No Hit
TTCACTGACCCGGTGAGGCGGGG1721660.24186486937286733No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG1647520.23144942066911375No Hit
TTTTTCACTGACCCGGTGAGGCGGGG1437900.20200126370552024No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1418740.1993095993251059No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC1411370.19827423572992567No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1411170.19824613902449337No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC1295620.18201326746098206No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1288430.1810031909006909No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCG1096720.1540710939085598No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1026460.14420072129019285No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1015410.14264837831505828No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1015360.1426413541387002No Hit
ATCGTATAGTGGTTAGTACTCTGC944660.13270916876838218No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG903560.12693529580204455No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT887570.12468896420273216No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC873970.12277838823333577No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAT867680.12189474684748995No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC839970.1180019483098448No Hit
CGACTGCATAATTTGTGGTAGTGGGG777720.10925684874404143No Hit
CGCCGTCGGGCCCGGGGGGCCGTCGTCACGCG761230.10694027538114832No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC735110.10327084565168994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)