FastQCFastQC Report
Wed 27 Sept 2023
ERR653353.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653353.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47639253
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC7800961.637506784583713No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4727960.9924504903550859No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG4573340.9599940620395538No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1954920.4103590793079816No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC1885860.3958626303397326No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC1822800.3826256469638598No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1719710.3609859289775178No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC1679870.35262307744414045No Hit
CTCGGTTGGCCCCGGATAGCCGGCTCCCCG1496320.31409392586403484No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1485390.31179959937658974No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGA1369090.28738695797769964No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG1350440.2834721190947305No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1150900.24158649171094262No Hit
TTTGGTGACTCTAGATAACCTCGGGCCG1123660.23586851792155514No Hit
TTGGTGACTCTAGATAACCTCGGGCCGA987450.20727654986529698No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG938290.19695732844509545No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT910620.19114909295492102No Hit
AGCTGCGAGAATTAATGTGAATTGCAGG893960.18765197682675672No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC891670.18717128079233314No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG890890.18700755026532428No Hit
AGCCAACAAGTACCGTAAGGGAAAGTTG888800.18656883641731326No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAT879370.18458937632796216No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGC813400.17074155213978692No Hit
TTTTCACTGACCCGGTGAGGCGGGG813280.1707163628279394No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATC778090.16332959712865355No Hit
ATCGTATAGTGGTTAGTACTCTGC774000.16247106141651718No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT745990.1565914562094414No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG708360.1486925078359226No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATC698020.14652202879839446No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTT665700.13973770747412853No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC613870.12885802386531964No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGAT537430.1128124322184481No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT514990.10810203090296147No Hit
TTGGTGACTCTAGATAACCTCGGGCCG513240.10773468677185177No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC507200.10646682474219317No Hit
TTTCACTGACCCGGTGAGGCGGGG484720.10174802698942403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)