FastQCFastQC Report
Wed 27 Sept 2023
ERR653354.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653354.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65575581
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC14702182.2420205472521855No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT8370201.2764202577175794No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG5125470.7816125944808633No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC3784790.5771645393427777No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG3524560.5374805600273675No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT2391650.364716555084735No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC2211260.3372078396072465No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC2168840.33073896821440285No Hit
CTCGGTTGGCCCCGGATAGCCGGCTCCCCG2167330.33050869957217766No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2136900.325868252696076No Hit
AGCTGCGAGAATTAATGTGAATTGCAGG2059730.31410015261626123No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2038100.3108016686882271No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAT1943640.2963969164070388No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC1616070.24644387062312112No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1465980.2235557775690924No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1410620.2151136106594313No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTT1405450.2143252074274416No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1247320.19021104822540572No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1235720.18844209706658946No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT1156790.1764056043971612No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1077190.16426693954873234No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1036230.15802071200863627No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1034870.15781331773484403No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1018430.15530628695459062No Hit
TTTTCACTGACCCGGTGAGGCGGGG961430.1466140269500624No Hit
ATCGTATAGTGGTTAGTACTCTGC914800.1395031482832001No Hit
GACAACCCGCAGTTTTATCCGGTAAAGC912350.13912953359879496No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGA864200.13178686133181192No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC855630.13047997241534162No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG853240.13011550747830963No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGCG815410.12434659175951487No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCG751260.1145639868596818No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCATTC741470.11307105307995668No Hit
AGCCAACAAGTACCGTAAGGGAAAGTTG739270.11273556234293981No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGC723040.11026055567849259No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG719670.10974664486769854No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATC698620.10653660849760524No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGC663060.10111385822109606No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)