FastQCFastQC Report
Wed 27 Sept 2023
ERR653355.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653355.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67138834
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC18516982.7580133429186455No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT8695901.2952116505329836No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG5492510.8180824230578685No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG5342230.7956989541998897No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT4636580.6905958480005774No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC3287830.4897061512864522No Hit
TTTTCACTGACCCGGTGAGGCGGGG3276050.48795157806881184No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC3207630.4777607546773898No Hit
AGCTGCGAGAATTAATGTGAATTGCAGG2218480.33043171408070626No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT2206590.3286607569026296No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1861250.27722405783812093No Hit
TTTCACTGACCCGGTGAGGCGGGG1824870.2718054352865288No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1815100.27035024170958943No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG1629970.24277603629517902No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTT1531580.2281213284103206No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGA1457980.21715896942744048No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG1454560.21664957720296424No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1410750.21012429259644277No Hit
AGCCAACAAGTACCGTAAGGGAAAGTTG1405690.20937063041636975No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAT1399950.208515685571781No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1378760.2053595390113567No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1192640.17763787795301897No Hit
TTGGTGACTCTAGATAACCTCGGGCCGA1164110.17338847439620414No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATC1153780.17184987156613413No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATT1131610.16854775881273124No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1118320.166568278501828No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGT1094650.163042748106111No Hit
ACAACCCGCAGTTTTATCCGGTAAAGC1077640.1605091920422687No Hit
TTTGGTGACTCTAGATAACCTCGGGCCG1069760.15933550469464514No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC1058630.15767774578867425No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1007650.15008452485189122No Hit
CTCGGTTGGCCCCGGATAGCCGGCTCCCCG984630.14665580876784365No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGC864740.12879878134314934No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC855340.1273986974513141No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC831990.12392082948595741No Hit
TTCACTGACCCGGTGAGGCGGGG830030.12362889710000027No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC815230.12142450969583415No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGC811120.12081234535589343No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC764050.11380149974007592No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG759450.11311635230364592No Hit
TTGGTGACTCTAGATAACCTCGGGCCG730070.10874034541618639No Hit
ATTCAAACGAGAACTTTGAAGGCCGAA727910.10841862401125406No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTT725450.10805221907785886No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT712060.10605784425746805No Hit
GACAACCCGCAGTTTTATCCGGTAAAGC687300.10236996370833608No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCCC687260.10236400590454102No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCT676630.1007807195460082No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCATTC672570.10017600246081128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)