FastQCFastQC Report
Wed 27 Sept 2023
ERR653361.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653361.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44318979
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTCTGGCGCCAAGCGCCCGTCCG14322643.2317170483552884No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC12674352.8598018920968373No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG6587931.4864805436966406No Hit
ATCGTATAGTGGTTAGTACTCTGC5935851.33934719028613No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC4241440.9570256571118211No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG3231570.7291616532953072No Hit
TCGACTGCATAATTTGTGGTAGTGGGG2783150.6279815245743815No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGG2749070.6202918167406338No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG2637680.5951581149917736No Hit
CGACTGCATAATTTGTGGTAGTGGGG2458150.5546495103147571No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC2299120.5187664634602707No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC2203980.49729936242439166No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC2184580.49292200526550933No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1883960.42509102026019147No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC1666640.3760555946020327No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT1574400.3552428407703165No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCG1524730.34403545262177637No Hit
GTCTAGGGGTATGATTCTCGCTT1314450.2965885112109645No Hit
GTCTAGCGGTTAGGATTCCTGGT1308430.2952301766699093No Hit
GTCTAGCGGTTAGGATTCCTGG1204770.2718406486755934No Hit
ATCGTATAGTGGTTAGTACTCTGCG1166680.26324613660436536No Hit
ACAACCCGCAGTTTTATCCGGTAAAGC1127950.2545072168742877No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1120540.2528352469491682No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC1060060.23918872318786946No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG1036840.23394943281522798No Hit
ACCTCTAGCATAACAAGTATTAGAGGC1010740.22806030797776275No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1010510.22800841147536363No Hit
GACTGCATAATTTGTGGTAGTGGGG993730.2242222231698975No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC972760.21949061597289957No Hit
GGGCCAGCTTTAGCTCAGCGGTTACTTCGACG968700.21857452988707163No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG949180.21417009629215508No Hit
GTGATCGTATAGTGGTTAGTACTCTGC859090.1938424619393872No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG792320.178776681655956No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG771560.17409245822201816No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC707750.15969456336076696No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT689120.15549094666643834No Hit
GACTGCATAATTTGTGGTAGTGGGGG684560.15446204209713404No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG675880.1525035132239847No Hit
ACGATTAAAGTCCTACGTGATCTGAGT670830.1513640465408736No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG623900.14077490368178383No Hit
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG616650.1391390356713768No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG612880.13828838430596518No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGG594730.13419307335577382No Hit
GACAACCCGCAGTTTTATCCGGTAAAGC593780.13397871823716878No Hit
CGCGGGGGCCGGGCCGCCCCTCCCACGGCG583190.13158922275713977No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC575270.12980217797887447No Hit
CTGGTGTAGTGGTATCATGCAAGATT560590.12648982730400896No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGC512080.11554417803713393No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATT509680.11500264931644746No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG495440.11178957890704115No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGC492780.111189384574947No Hit
GTGTAGTGGTATCATGCAAGATT490740.1107290851623635No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG484770.10938203246965594No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC471060.10628854965273454No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCG466270.10520774858103117No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC458700.1034996767411993No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC457410.10320860505383034No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATT448260.1011440268062132No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG446610.10077172581074126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)