FastQCFastQC Report
Wed 27 Sept 2023
ERR653362.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653362.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56938698
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC14452722.5382947815209964No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG6259111.099271711481706No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC3858540.677665653682492No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT2612680.45885840241728043No Hit
ACGATTAAAGTCCTACGTGATCTGAGT2526080.44364906271653776No Hit
TCAAAATGTAAACTTAAAATATAGCCAAA2136790.37527904132967704No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG1794530.3151687802906909No Hit
AAATATGAACTAAAAAATTTCGGTTGGG1710900.3004810542032415No Hit
GCGTGCTTTAAGGAAAGATTAAAAAAAGT1659070.29137828195509496No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC1578410.27721216948093896No Hit
GCGTGCTTTAAGGAAAGATTAAAAAAAGTA1441730.2532074056206905No Hit
AAAGCATCTGGCCTACACCCAGAAGAA1338560.23508791858921677No Hit
GCGTGCTTTAAGGAAAGATTAAAAAAAG1282160.2251825287610194No Hit
ATCGTATAGTGGTTAGTACTCTGC1278290.22450285041642504No Hit
ACGTGCCAACTATAAATCTCATAATAGAATT1263540.22191234509788055No Hit
AGGTCGGTTTCTATCTATTTACAATTTCTC1238280.21747599497269854No Hit
TCAATCAGTGAAATTGACCTTCCAGTGA1200650.21086713292952358No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1200290.21080390703700322No Hit
ACAACCCGCAGTTTTATCCGGTAAAGC1148630.20173099146032458No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC1093360.19202406068364963No Hit
CGTGCCAACTATAAATCTCATAATAGAATT1082160.19005703291634804No Hit
ACAAAGCATCTGGCCTACACCCAGAAGAA1049940.18439831553577146No Hit
GGGCATCAAGCACATAATATAGCTCAAGACGCC976930.1715757532776742No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG943040.1656237380067946No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG898870.15786627224949892No Hit
TCAAAATGTAAACTTAAAATATAGCCAAAA884690.15537587459411173No Hit
TAAATATGAACTAAAAAATTTCGGTTGGG864990.15191601325341159No Hit
TCAAAATGTAAACTTAAAATATAGCCAA813680.14290456729446113No Hit
TTCAATCAGTGAAATTGACCTTCCAGTGA782420.137414452293939No Hit
AGGCGTGCTTTAAGGAAAGATTAAAAAAAGTA775580.13621316033605124No Hit
TTTAACCTAGACTCACAAGTCAAAGTAA774800.13607617090225704No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG746500.13110591324023602No Hit
TCGACTGCATAATTTGTGGTAGTGGGG744360.13073007043469803No Hit
GCGTGCTTTAAGGAAAGATTAAAAAAA743700.1306141562984106No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC740050.12997311599924538No Hit
AACGATTAAAGTCCTACGTGATCTGAGT722930.12696637355494148No Hit
AGGCGTGCTTTAAGGAAAGATTAAAAAAAGT716040.12575630022309256No Hit
ACAAAGCATCTGGCCTACACCCAGAAGAAT705210.12385425462310361No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC700620.12304812449346839No Hit
AATATGAACTAAAAAATTTCGGTTGGG687160.120684178623122No Hit
CGCTCGCCGAATCCCGGGGCCGAGGGAGCCG658550.11565947644254176No Hit
TTAACCTAGACTCACAAGTCAAAGTAA630540.11074015074949554No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT621830.10921043540546009No Hit
TCAAAATGTAAACTTAAAATATAGCCAAAAG616520.10827785349078406No Hit
AGGGCATCAAGCACATAATATAGCTCAAGACGCC609480.10704143603705164No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG592690.10409265066089148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)