FastQCFastQC Report
Wed 27 Sept 2023
ERR653363.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653363.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58443014
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC13769292.356019831557626No Hit
TCGACTGCATAATTTGTGGTAGTGGGG5412440.9261055564314326No Hit
ATCGTATAGTGGTTAGTACTCTGC4469880.7648270843800082No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG4224190.7227878425298189No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG3986090.6820473016672275No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG3950050.6758806108117559No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG3806430.6513062450885918No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC3701590.6333674029884906No Hit
CGACTGCATAATTTGTGGTAGTGGGGG3325760.5690603157462071No Hit
CGCCGTCGGGCCCGGGGGGCCGTCGTCACGCG2368110.4051998413360406No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG2170460.37138057253515366No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG1948990.3334855385795127No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC1900020.3251064361601884No Hit
GTCTAGGGGTATGATTCTCGCTT1784890.3054069045788775No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC1759220.30101459175257456No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1718200.29399578878666316No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG1678740.28724391250594977No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG1657560.28361986943383855No Hit
ATCGTATAGTGGTTAGTACTCTGCG1626280.2782676471819198No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC1591340.2722891738608827No Hit
GGGCCAGCTTTAGCTCAGCGGTTACTTCGACG1439160.2462501335061193No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG1387360.23738679870275No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG1353500.23159312077915764No Hit
CGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG1336130.22862099480358763No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG1319000.22568993447189428No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1230790.21059659927874355No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC1190490.20370099324446203No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC1174440.2009547283102134No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1158780.19827519504726435No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1143790.19571030337347078No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT1106580.18934341750410066No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1087550.1860872541583841No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA1083440.1853840050069971No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1066000.18239990155196306No Hit
CGACTGCATAATTTGTGGTAGTGGGG995910.17040702247149675No Hit
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG946510.16195434410689358No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA928570.15888468722711666No Hit
GACAACCCGCAGTTTTATCCGGTAAAGC920390.15748503319832205No Hit
CTGGTGTAGTGGTATCATGCAAGATT908420.1554368842099759No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG788480.13491432868263778No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG756250.1293995549237074No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG750290.12837975810077146No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG730110.12492682187814613No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG728600.12466845053542243No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGA715990.1225107931634053No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC702160.12014438543501538No Hit
GACTGCATAATTTGTGGTAGTGGGGG692860.11855309173479656No Hit
GTCTAGCGGTTAGGATTCCTGGT657550.11251130887944966No Hit
CTCGGTTGGCCCCGGATAGCCGGCTCCCCG652210.11159759830319496No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG639180.10936807605439378No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG634460.10856045172482034No Hit
ATCGTATAGTGGTTAGTACTCTGCGT628950.10761765298415307No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC615280.1052786223516809No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGC599330.1025494681023809No Hit
GTCTAGCGGTTAGGATTCCTGG597660.10226371966373946No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT588420.10068269237448978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)