FastQCFastQC Report
Wed 27 Sept 2023
ERR653364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58074250
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC13343722.297699927248307No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG4020980.6923860402846357No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG3661560.6304963043000986No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG3086430.5314627395101961No Hit
TCGACTGCATAATTTGTGGTAGTGGGG2861140.49266929835512296No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC2079530.3580812494349906No Hit
ATCGTATAGTGGTTAGTACTCTGC1937770.3336711192998618No Hit
CGCCGTCGGGCCCGGGGGGCCGTCGTCACGCG1934600.33312526636159745No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG1776210.30585156071752967No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1609550.2771538160200088No Hit
CGACTGCATAATTTGTGGTAGTGGGG1596690.27493940946288586No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1553620.267523041623439No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1553210.26745244234751203No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC1485440.2557828986168569No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC1438440.2476898108886469No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1426940.24570958729557418No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC1352630.23291389901720644No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1326640.2284385936968622No Hit
GACAACCCGCAGTTTTATCCGGTAAAGC1118770.19264476080190446No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG977870.16838271695286638No Hit
CGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG974860.16786441495154908No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC947200.16310154672681954No Hit
ACAACCCGCAGTTTTATCCGGTAAAGC897650.15456936594101517No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG847740.14597519554707983No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC829780.14288260287476806No Hit
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG824610.141992363224665No Hit
ACGATTAAAGTCCTACGTGATCTGAGT820110.14121749312302784No Hit
ATCGTATAGTGGTTAGTACTCTGCG818700.14097470049118155No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG797540.13733108907992786No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC788010.13569008639801633No Hit
GGGCCAGCTTTAGCTCAGCGGTTACTTCGACG782930.13481534414994598No Hit
AACGATTAAAGTCCTACGTGATCTGAGT770870.13273869227755847No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG751080.1293309857639143No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGCG704960.12138942818891332No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGC653280.1124904755550007No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG587930.10123763974567042No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGG585080.10074688868130022No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG582120.10023719634777893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)