FastQCFastQC Report
Wed 27 Sept 2023
ERR653365.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653365.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69169933
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC13007141.8804615583479025No Hit
CGACTGCATAATTTGTGGTAGTGGGG5810290.8400022593631831No Hit
TCGACTGCATAATTTGTGGTAGTGGGG5537880.8006195408632245No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG4934560.7133966719325866No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG4346290.628349603866177No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG3440510.49739964328142405No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC2772910.40088371923101324No Hit
CGACTGCATAATTTGTGGTAGTGGGGG2577670.372657582305306No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT2571560.37177425052587515No Hit
ATCGTATAGTGGTTAGTACTCTGC2075950.3001231763517828No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG2025670.29285412203594297No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG1878620.27159488502034546No Hit
CGCCGTCGGGCCCGGGGGGCCGTCGTCACGCG1857030.2684735866377086No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC1839980.26600864280149006No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC1787950.2584865883851586No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1690130.2443446056251059No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG1688200.24406558265713515No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1564880.22623702700420426No Hit
GGGCCAGCTTTAGCTCAGCGGTTACTTCGACG1480510.21403953073078735No Hit
ACAACCCGCAGTTTTATCCGGTAAAGC1454590.21029223781379114No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC1372790.1984662902593819No Hit
GACAACCCGCAGTTTTATCCGGTAAAGC1365550.1974195927007765No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC1191980.1723263198765857No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG1153990.16683404912362718No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1127670.1630289276122329No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG1084810.15683259372247765No Hit
ACGATTAAAGTCCTACGTGATCTGAGT1073780.15523797023195035No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC1068470.15447029564131573No Hit
CACAAAGCATCTGGCCTACACCCAGAAGAA1068090.1544153584766375No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT1006240.145473612067833No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGC981430.1418867935002915No Hit
GGGCATCAAGCACATAATATAGCTCAAGACGCC977650.14134031328322957No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC919740.13296817852924622No Hit
AAAGCATCTGGCCTACACCCAGAAGAA905170.13086177197829582No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC868370.12554154129367162No Hit
CGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG845570.12224531141298055No Hit
GACTGCATAATTTGTGGTAGTGGGGG835360.12076923654096931No Hit
ACAAAGCATCTGGCCTACACCCAGAAGAAT832630.12037455638420236No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA831630.12022998489820715No Hit
ACAAAGCATCTGGCCTACACCCAGAAGAA789630.11415798248640778No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC767780.11099909551741216No Hit
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG763610.11039623242081209No Hit
GACTGCATAATTTGTGGTAGTGGGG745640.10779828281747793No Hit
CTGGTGTAGTGGTATCATGCAAGATT733840.1060923392827343No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA728720.10535213327443876No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG727360.10515551605348526No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA725600.10490107023813366No Hit
CACAAAGCATCTGGCCTACACCCAGAAGAAT717360.10370980119353303No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG696150.10064343997557436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)