FastQCFastQC Report
Wed 27 Sept 2023
ERR653371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51830819
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC22476604.336531900065094No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG20475353.9504199229419856No Hit
ATCGTATAGTGGTTAGTACTCTGC7936741.5312781378198943No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC4544250.8767467093275142No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC4533740.8747189582321669No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC4041710.7797889514344738No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG3864370.7455737869008012No Hit
TCGACTGCATAATTTGTGGTAGTGGGG3674360.7089141307992837No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG3044130.5873204511778987No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT2933840.5660416054780072No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC2596270.5009124011719746No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG2389870.46109053379997716No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGG2359390.45520986268806596No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC2224330.429152007032727No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG2141990.41326570587279354No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1706600.3292635603539277No Hit
ATCGTATAGTGGTTAGTACTCTGCG1653660.3190495600696566No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1597940.3082991993624488No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG1596430.3080078669025083No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC1326770.2559809058776401No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG1299450.2507099106421606No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCG1235900.23844886572214882No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG1164820.2247350172105133No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGG1152330.22232525401537645No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGT1025000.19775878903244806No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1010190.1949014157001841No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC1006480.19418562535158862No Hit
GTCTAGGGGTATGATTCTCGCTT918720.17725361430233236No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG902420.1741087672182066No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC896540.17297430704307412No Hit
GACAACCCGCAGTTTTATCCGGTAAAGC852660.164508301518446No Hit
CCCGGGGAGCCCGGCGGGTCGCCGGCGCGG836140.16132100864545473No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT830440.1602212768430304No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC823080.15880127227007546No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATT821270.15845205918895475No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGC813050.15686613016861647No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG781420.15076358334218104No Hit
CGACTGCATAATTTGTGGTAGTGGGG777710.15004779299358553No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC717120.13835783686921868No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCG715490.13804335216080613No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGCG700300.13511266337504718No Hit
CTGGTGTAGTGGTATCATGCAAGATT698370.1347402980454544No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG685150.13218969200544564No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATT684840.13212988203022608No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT675700.13036645243826844No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG671820.12961786307100415No Hit
GGCGACCGGCCGCCGCCGGGCGCACTTCCACCGT665820.12846025064739958No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCG654670.1263090208935344No Hit
GTCTAGCGGTTAGGATTCCTGG654500.12627622187486562No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCG637080.12291528713833366No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG626750.12092226441569447No Hit
GTGATCGTATAGTGGTTAGTACTCTGC624830.12055182844014098No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG619230.11947139017811005No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC599140.11559531791307408No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTC574480.11083753085205927No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTATGGG564680.10894676389350513No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGC562460.10851844729677143No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGG560400.10812100036466721No Hit
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG557670.10759428671192713No Hit
CTCGGTTGGCCCCGGATAGCCGGCTCCCCG550910.10629004338133265No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCG544390.10503210454768233No Hit
GACTGCATAATTTGTGGTAGTGGGGG544010.10495878909418738No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGG543130.10478900593872537No Hit
ATCATCACCCACGGAGAAGAGAAGGACTG542760.10471761983926976No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAG536490.10350791485660299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)