FastQCFastQC Report
Wed 27 Sept 2023
ERR653372.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653372.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61797665
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG14957002.4203179845063727No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG13683942.214313437247184No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC12326751.9946951070076837No Hit
TCGACTGCATAATTTGTGGTAGTGGGG7066661.143515697559123No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG6023500.9747132031606696No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG5497760.8896387913685735No Hit
ATCGTATAGTGGTTAGTACTCTGC5354510.8664583038857537No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA4924610.7968925686755317No Hit
CGACTGCATAATTTGTGGTAGTGGGGG4743980.7676633089615926No Hit
CGCCGTCGGGCCCGGGGGGCCGTCGTCACGCG4295970.6951670423146247No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGA3849800.6229685215452719No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC3802620.615333928879028No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC3448060.5579595928098577No Hit
ATCGTATAGTGGTTAGTACTCTGCG3393400.5491145984237431No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG3372160.5456775753582275No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG3292730.5328243389131289No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC3163280.5118769455124235No Hit
CTGGTGTAGTGGTATCATGCAAGATT3007210.48662194599100145No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT2910210.4709255600515003No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG2857990.4624754025900494No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG2732300.4421364464175143No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT2596210.420114578762806No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC2505290.4054020487667293No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG2489930.4029165179622887No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA2446980.3959664171777364No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG2418520.39136106517940444No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG2404730.3891295892814073No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG2312000.37412416796006775No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG2249220.3639652080705639No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA2114820.3422168135317087No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC2086420.33762117063808156No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG2050900.3318733806528127No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1992760.3224652581938169No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG1860250.30102270045316437No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC1851780.2996520985056636No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG1804090.29193497844942845No Hit
GTGGTCTAGTGGTTAGGATTCGGCG1783930.2886727192686002No Hit
ATCGTATAGTGGTTAGTACTCTGCGT1769680.28636680690119926No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG1764190.2854784238207059No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT1756380.2842146220249584No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTATGGG1727280.27950570624310805No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGCG1688930.27329997015259394No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG1547940.2504851922803232No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1435470.2322854755110893No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1429410.23130485593589986No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT1411840.22846170644149744No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC1375530.2225860799109481No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA1341200.21703085383565868No Hit
CTCGGTTGGCCCCGGATAGCCGGCTCCCCG1285530.208022422853679No Hit
GTCTAGGGGTATGATTCTCGCTT1281350.20734602189257473No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG1243270.20118397677323244No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT1236180.20003668423394314No Hit
CGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG1217420.19700097082956128No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1166810.18881134101102365No Hit
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG1129070.18270431415167546No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGG1127620.1824696774546417No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGG1045980.16925882231958117No Hit
CGACTGCATAATTTGTGGTAGTGGGG1027780.16631372722577786No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1022230.1654156350405796No Hit
AGGCGGCCCGGGTTCGACTCCCGGTATGGG1019140.16491561614828004No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC976550.15802376999195683No Hit
GTCTAGCGGTTAGGATTCCTGG950190.15375823665829447No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT949970.15372263660771002No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGA886600.14346820385527512No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA883060.14289536667768918No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCG856460.13859099692520746No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG843030.13641777565543942No Hit
GTCTAGCGGTTAGGATTCCTGGT805440.13033502155785334No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGC757010.12249815587692514No Hit
GACAACCCGCAGTTTTATCCGGTAAAGC693870.11228094135919213No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC674450.10913842780305696No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGG672420.10880993642720968No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGC657330.10636809659394089No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATT648350.10491496725644892No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGA643800.1041786934829981No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG638010.10324176487898046No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT636120.10293592808077781No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGA628890.10176598096384384No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTATGGGA628810.10175303549090407No Hit
GACTGCATAATTTGTGGTAGTGGGGG620670.10043583361927994No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCG619940.10031770617870432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)