FastQCFastQC Report
Wed 27 Sept 2023
ERR653373.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653373.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58832230
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC10826221.8401852182043754No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG9960811.6930872754610866No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG8640761.4687119628135803No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG6544131.1123375741494075No Hit
ATCGTATAGTGGTTAGTACTCTGC6344481.0784020935463436No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC4610440.7836588890137259No Hit
CGACTGCATAATTTGTGGTAGTGGGGG4443730.7553223802667347No Hit
ATCGTATAGTGGTTAGTACTCTGCG4378760.7442791136762961No Hit
TCGACTGCATAATTTGTGGTAGTGGGG4182030.7108399596615664No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC3961830.6734114957056702No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG3718580.6320651112493951No Hit
CGCCGTCGGGCCCGGGGGGCCGTCGTCACGCG3678920.6253239083407174No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG3563330.6056765143867571No Hit
CGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG3026200.514377918362095No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC2904850.49375146922018764No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG2892240.4916080862479631No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA2788270.473935800155799No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG2752300.4678218044768998No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT2667280.45337054196313825No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC2485660.42249970806817966No Hit
ATCGTATAGTGGTTAGTACTCTGCGT2337990.3973995206369026No Hit
GTCTAGGGGTATGATTCTCGCTT2325740.39531732861392466No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG2038760.3465379435727662No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGA1879660.31949494350290647No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG1867720.31746544368622437No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG1672950.28435944039517114No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG1650710.2805791995305974No Hit
CTGGTGTAGTGGTATCATGCAAGATT1618280.27506691485262413No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG1599750.2719172807150094No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT1559810.2651284848458064No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA1546090.26279642978007123No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1491910.2535871919184433No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1447260.24599781446326274No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTC1411010.2398362258238384No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1395240.23715572229711504No Hit
GTCTAGCGGTTAGGATTCCTGG1337250.22729888022262626No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG1335090.2269317345271461No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT1326340.22544445451073333No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG1171830.19918163904376904No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1144480.19453282664961025No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1141960.19410449000488336No Hit
GTGGTCTAGTGGTTAGGATTCGGCG1102600.18741427955391118No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG1094700.1860714781676642No Hit
GTCTAGCGGTTAGGATTCCTGGT1043070.17729567619653377No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTATGGG1038660.17654608706826172No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC1034890.1759052818497616No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGG999600.1699068690749951No Hit
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG984530.16734534794958478No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGC929160.15793384000572475No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC918490.15612020825999628No Hit
CGTGCTTTAAGGAAAGATTAAAAAAAGT903370.153550188391635No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGG880530.1496679626116501No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGCG876360.14895916744954255No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG868100.1475551751140489No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGC864280.14690587115259782No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG843300.14333979861038754No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGC816670.13881336811472214No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG809310.13756235315234522No Hit
CGGTCGCGGGGGCCGGGCCGCCCCTCCC790390.1343464288197133No Hit
AGGCGGCCCGGGTTCGACTCCCGGTATGGG787270.13381610725957524No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG786230.13363933340619588No Hit
CAGTGCGCCCCGGGCGTCGTCGCGC781880.13289994276946496No Hit
CGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGC775430.13180360492879498No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT766640.13030952591802147No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG758500.12892593056561003No Hit
CGTGCTTTAAGGAAAGATTAAAAAAAGTA740640.1258901795835378No Hit
GACAACCCGCAGTTTTATCCGGTAAAGC728270.12378759057747768No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC722130.12274394494310346No Hit
AAAGGACTTGGCGGTACTTTATATCCATCTAGA711780.12098470515226094No Hit
CGACTGCATAATTTGTGGTAGTGGGG667630.11348031512658963No Hit
GGGCCAGCTTTAGCTCAGCGGTTACTTCGACG652230.11086270229770316No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGA641840.10909666351249987No Hit
GACTGCATAATTTGTGGTAGTGGGGG636790.10823829047445592No Hit
CGTGATCGTATAGTGGTTAGTACTCTG592970.10078999215226075No Hit
AAAGCATCTGGCCTACACCCAGAAGAAT590230.10032426103854979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)