FastQCFastQC Report
Wed 27 Sept 2023
ERR653374.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653374.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49408427
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length20-48
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC13876342.8084966153648243No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG5685021.1506174847460737No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG3697720.7483986486758626No Hit
TCGACTGCATAATTTGTGGTAGTGGGG3514380.7112916183306139No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG3199850.6476324372763376No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG2934700.5939675027500876No Hit
CGACTGCATAATTTGTGGTAGTGGGGG2878210.5825342304461545No Hit
CGCCGTCGGGCCCGGGGGGCCGTCGTCACGCG2575790.5213260482872689No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT2390710.48386685129643975No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC2116890.4284471553810041No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC2085090.42201100634108424No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG2023390.40952325804664863No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1894110.38335768106926377No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC1678770.33977402275931595No Hit
ATCGTATAGTGGTTAGTACTCTGC1616480.3271668616367811No Hit
CGACTGCATAATTTGTGGTAGTGGGG1603010.32444060605289055No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1357520.2747547498324526No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC1244110.2518011755363109No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG1133090.22933132439128248No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC1128700.22844281199237532No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGC1102830.22320686307216378No Hit
ATCGTATAGTGGTTAGTACTCTGCG1077820.21814497352850354No Hit
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG1018530.20614499627765928No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT988470.20006101388331995No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC954790.193244362950474No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG950600.1923963294763462No Hit
GACAACCCGCAGTTTTATCCGGTAAAGC943210.1909006332057485No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG942830.19082372324866767No Hit
CGTGCTTTAAGGAAAGATTAAAAAAAGT911850.18455353780034325No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA907070.1835860914981163No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGG905470.18326226009988134No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGG903130.18278865667996272No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC895760.18129700830184292No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG859450.1739480595081483No Hit
CGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG855610.1731708641523844No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA821030.16617205805803129No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC812890.16452456581951092No Hit
AAAGCATCTGGCCTACACCCAGAAGAA810260.1639922679586622No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG759660.15375109998948155No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG749070.15160774092241389No Hit
GACTGCATAATTTGTGGTAGTGGGGG724290.14659240214224994No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG715980.1449105028176671No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT691480.13995183453219426No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT690570.13976765542444816No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC667180.1350336451715008No Hit
CGTGCTTTAAGGAAAGATTAAAAAAAGTA662810.13414918066507156No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG662420.13407024676175178No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC642810.13010128818713454No Hit
AAAGCATCTGGCCTACACCCAGAAGAAT616430.12476211800873564No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC610270.12351536712553103No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGA576630.11670681197764098No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG573180.11600855052519685No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA571310.11563007257850973No Hit
CGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGC559620.11326407942515554No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG553240.11197280172469365No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG548280.11096892439016526No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT547460.11080296079856984No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC545560.11041841101316584No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCG544170.11013708248594921No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG534220.10812325597817554No Hit
AGGCGGCCCGGGTTCGACTCCCGGTATGGG531810.10763548493458414No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTC525180.10629360857814803No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGCG520830.10541319196419673No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGC500700.10133898818515312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)