FastQCFastQC Report
Wed 27 Sept 2023
ERR653375.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR653375.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67813880
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length20-49
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC11126351.6407186847294388No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG8751731.2905514328335144No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGT8678471.2797483347067002No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG8148781.2016389565086085No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT6863861.0121615220954765No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG6622430.976559665956291No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC6566410.9682988202415199No Hit
TCGACTGCATAATTTGTGGTAGTGGGG6353380.9368848973101082No Hit
CGACTGCATAATTTGTGGTAGTGGGGG5741950.846721939520346No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG5067360.7472452542163934No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC4875520.718956060322754No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG4856180.7161041367932347No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC4701470.693290223181449No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA4398430.6486032063052579No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG4255650.627548519565611No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG4136810.6100240835651934No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG3977360.5865111980025328No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCG3785480.5582161056114177No Hit
ATCGTATAGTGGTTAGTACTCTGCG3661760.53997205291896No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG3318460.4893481983334385No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA2990650.44100853689539665No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG2888540.4259511474642064No Hit
ATCGTATAGTGGTTAGTACTCTGC2754940.4062501658952415No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGC2706120.3990510497261033No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGA2687930.3963687079990114No Hit
CTGGTGTAGTGGTATCATGCAAGATT2622430.3867099183824904No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG2473340.36472474366604596No Hit
CGCCGTCGGGCCCGGGGGGCCGTCGTCACGCG2239260.3302067364380271No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG2100710.3097758158064396No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG1889870.2786848356118246No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA1863400.27478150490725495No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGG1776960.2620348518621851No Hit
GTCTAGGGGTATGATTCTCGCTT1732540.2554845704153781No Hit
CGACTGCATAATTTGTGGTAGTGGGG1679480.24766021351381165No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG1671840.24653360049594567No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1516630.22364595566571327No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1497650.2208471186134756No Hit
ATCGTATAGTGGTTAGTACTCTGCGT1485720.21908789174133672No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAG1417910.20908846389559188No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGC1363140.20101194622693763No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGA1306080.19259773957779736No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC1217830.17958417952195038No Hit
ATTGTTAGGACCTAAGCCCAATAACGAAAGT1190370.17553486100485624No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1165020.17179668822960728No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG1151510.16980447070717675No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT1132200.16695697105076424No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1132030.16693190243649236No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1129700.1665883149585306No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1119400.16506945185852806No Hit
CGTGATCGTATAGTGGTTAGTACTCTG1113090.1641389638817304No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1101990.16250213083221313No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT1069920.15777301048104017No Hit
CTCGGTTGGCCCCGGATAGCCGGCTCCCCG1068380.15754591832822423No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCATTC1046000.15424570899054885No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATT1040810.15348037894307182No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGC1014480.14959769297966727No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1011720.14919069665384135No Hit
GACTGCATAATTTGTGGTAGTGGGGG1009760.1489016702775302No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG990810.14610725709839933No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA986790.14551445810208766No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC922300.13600460554682905No Hit
TCGACAACCCGCAGTTTTATCCGGTAAAGC909310.13408906849158314No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG894360.13188450506002605No Hit
CGACAACCCGCAGTTTTATCCGGTAAAGCG887940.1309377962151701No Hit
GGCGACCGGCCGCCGCCGGGCGCACTTCCACCGT880890.12989818603507128No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG874050.1288895429667201No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGG869190.1281728755234179No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT829810.12236580475855384No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGG808890.11928089057874289No Hit
CCGTGATCGTATAGTGGTTAGTACTCTG802410.11832533398767334No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTATGGG799470.11789179442320659No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA783710.1155677864177658No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG763320.11256102732950836No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGG752800.11100972249338927No Hit
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCG743840.10968845905882396No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTC743370.10961915171348402No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG737810.10879926056435645No Hit
CGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGC732580.1080280320194037No Hit
CGTGCTTTAAGGAAAGATTAAAAAAAGT731950.10793513068416082No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCT720740.10628207676658524No Hit
GTGATCGTATAGTGGTTAGTACTCTGC720610.1062629066497891No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACT704600.10390203303512496No Hit
GACAACCCGCAGTTTTATCCGGTAAAGC686940.10129784639958664No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGC682460.10063721468230398No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGA681900.10055463571764364No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCG678850.10010487528511862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)