FastQCFastQC Report
Sun 2 Jul 2023
ERR6548319_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6548319_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108804256
Total Bases10.8 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCA1362412612.521684813505823No Hit
GAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAACT25196532.3157669494105084RNA PCR Primer, Index 1 (100% over 25bp)
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCCTGGAATTCTCGGGTGCC21265621.9544842069413166No Hit
CCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGTGGAATTCTCGGGTG17387211.598026643369539No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA15966861.467484874856366RNA PCR Primer, Index 1 (100% over 22bp)
CCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGT15315201.4075919971365825No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGTGGAATTCTCGG14902961.3697037733523953No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG10597140.9739637390655013No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCTGGAATTCTCGGGTGC9659990.8878319980424295No Hit
CCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGGA8528850.7838709912229904RNA PCR Primer, Index 1 (100% over 22bp)
AGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTC7507320.6899840388596564RNA PCR Primer, Index 1 (100% over 26bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG7357080.6761757554778004Illumina Small RNA Adapter 2 (100% over 21bp)
GAGGTGTAGAATAAGTGGGAGGCCCCTGGAATTCTCGGGTGCCAAGGAAC7206300.6623178416844283RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA5967410.5484537296041067RNA PCR Primer, Index 1 (100% over 22bp)
CGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGTGGAATTCTCGGGTGCC5823750.5352502019773933No Hit
CGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGGAA5816420.5345765150951448RNA PCR Primer, Index 1 (100% over 23bp)
GACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTGGAATTCTCGGGTGC5778650.5311051435340911No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGG5528250.5080913378976646Illumina Small RNA Adapter 2 (100% over 21bp)
GCTGAGAAGACGGTCGAACTTGACTATCTAGAGTGGAATTCTCGGGTGCC5412150.4974207994216696No Hit
CGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTGGAATTCTC5241030.48169347346118513No Hit
AGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAAC5073350.46628231160369316RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTGGAATTCTC4716990.4335299163297435No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTG4339560.3988410159249653No Hit
TTCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGGTGCCAAGGAA4317260.3967914637456829RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCTGGAATTCTCGGGTGC4064320.37354421135879096No Hit
GAGGTGTAGAATAAGTGGGAGGCCCCCTGGAATTCTCGGGTGCCAAGGAA4043270.37160954439135174RNA PCR Primer, Index 1 (100% over 23bp)
CGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCATGGAATTCTCG4006530.36823283824485686No Hit
GGTTCTATTTTGTTGGTTTTCGGAACTGGAATTCTCGGGTGCCAAGGAAC3505980.32222820401437235RNA PCR Primer, Index 1 (100% over 24bp)
CCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGG3473780.31926876095729195No Hit
CCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACTGGAATTCTCGGG3229350.2968036470926284No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGGTGGAATTCTCG2969430.2729148756828042No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC2942120.2704048635744543RNA PCR Primer, Index 2 (100% over 50bp)
AGGTGTAGAATAAGTGGGAGGCCCCTGGAATTCTCGGGTGCCAAGGAACT2938250.27004917895858777RNA PCR Primer, Index 1 (100% over 25bp)
CCCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGG2663280.2447771895981716No Hit
TCACCAAGCGTTGGATTGTTCACCCACTAATAGGTGGAATTCTCGGGTGC2639060.24255117373349808No Hit
CGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGA2295700.21099358466271761RNA PCR Primer, Index 1 (100% over 22bp)
CGGACTGTCCCCAGTGCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2262420.2079348807826047RNA PCR Primer, Index 1 (100% over 30bp)
AGGTGTAGAATAAGTGGGAGGCCCCCTGGAATTCTCGGGTGCCAAGGAAC2219520.2039920203121466RNA PCR Primer, Index 1 (100% over 24bp)
CGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCTGGAATTCTCGGGTGCCA2206690.20281283849778817No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG2187100.2010123574577818Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGG2168920.19934146693673452Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA2166560.1991245636567746RNA PCR Primer, Index 1 (100% over 22bp)
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGGAATTCTCGGGT2065020.1897922081283291No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGAGTGGAATTCTCGGGTGC2010980.18482549064992457No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1956370.17980638551491956Illumina Small RNA Adapter 2 (100% over 21bp)
GGATTGGCTCTAAGGGCTGGGTCGGTCTGGAATTCTCGGGTGCCAAGGAA1955770.17975124061323483RNA PCR Primer, Index 1 (100% over 23bp)
CCCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGG1884150.17316877751546778No Hit
CCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGTGGAATTCTCGGGTGCC1842760.16936469838091628No Hit
TCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGGTGCCAAGGAAC1835510.1686983641522258RNA PCR Primer, Index 1 (100% over 24bp)
TTCTATTTTGTTGGTTTTCGGAACTGGAATTCTCGGGTGCCAAGGAACTC1810850.1664319086929835RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGAA1797290.16518563391490862RNA PCR Primer, Index 1 (100% over 23bp)
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTGGAATTCTCGGGTGCCA1757050.16148724917525284No Hit
CGGTTCTATTTTGTTGGTTTTCGGAACTGGAATTCTCGGGTGCCAAGGAA1711140.1572677451146764RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCA1653430.1519637246543003No Hit
CCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCTGGAATTCTCGGGTGCC1561900.143551369902295No Hit
GTGTAATGGTTAGCACTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1560060.14338225887046183RNA PCR Primer, Index 1 (100% over 30bp)
GGCTTTGGTCTAGGGGTATGATTCTCTGGAATTCTCGGGTGCCAAGGAAC1557340.14313226864949108RNA PCR Primer, Index 1 (100% over 24bp)
TTTCGGAACTGAGGCCATGATTAAGAGTGGAATTCTCGGGTGCCAAGGAA1548690.14233726298353622RNA PCR Primer, Index 1 (100% over 23bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGGAATTCTCGGGTGCC1487560.13671891658355717No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGGTGCCAAGGA1474340.13550389058310366RNA PCR Primer, Index 1 (100% over 22bp)
GCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGTGGAATTCTCGGGTG1468880.13500207197777264No Hit
CCCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGTGGAATTCTCGGGT1438420.13220254913557794No Hit
GAGCGCTGAGAAGACGGTCGAACTTGACTGGAATTCTCGGGTGCCAAGGA1420320.13053901126808864RNA PCR Primer, Index 1 (100% over 22bp)
CCGCGGCGGGGCGCGGGACATGTGGCGTACGGAATGGAATTCTCGGGTGC1345850.12369460988731912No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCC1330910.12232150183536937No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGTGGAATTCTCGGG1292560.1187968235360205No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1270810.1167978208499491No Hit
TTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGCCAAGGAAC1261980.11598627171348885RNA PCR Primer, Index 1 (100% over 24bp)
GCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTGGAATTCTCG1252080.11507638083569086No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCTGGAATTCTCGG1251560.11502858858756407No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA1242660.1142106058792406RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA1239060.11387973646913223RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCTGGAATTCTCGGGTG1209740.11118498894013852No Hit
CCGAGGTGGGATCCCGAGGCCTCTCCAGTGGAATTCTCGGGTGCCAAGGA1184260.10884316878192707RNA PCR Primer, Index 1 (100% over 22bp)
GTCACGCACCGCACGTTCGTGGGGAACCTGTGGAATTCTCGGGTGCCAAG1162220.10681751272670806No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA1161350.1067375526192652RNA PCR Primer, Index 1 (100% over 22bp)
AGAGGTGTAGAATAAGTGGGAGGCCCCTGGAATTCTCGGGTGCCAAGGAA1141350.10489938922977424RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCA1127250.10360348404018313No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA1119450.10288660031828167No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAA1118860.10283237449829168No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAG1117410.1026991076525536No Hit
CCTCTGGCATGTTGGAACAATGTAGGTAAGGTGGAATTCTCGGGTGCCAA1112120.10221291343603324No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGTGGAATTCTCGGGTG1109590.10198038576726264No Hit
CGCGGTTCTATTTTGTTGGTTTTCGGAACTGGAATTCTCGGGTGCCAAGG1098800.10098869661863227Illumina Small RNA Adapter 2 (100% over 21bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGTGGAATTCTCGGGTGCCAAGG1090980.10026997473334132Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)