FastQCFastQC Report
Mon 26 Jun 2023
ERR6548320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6548320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20001290
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length87-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGTGGAATTCTCGG448975322.44731714804395No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGTGGAATTCTCG7913133.956309818016738No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGGTGGAATTCTCG5091232.5454508184222115No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCA4507942.253824628311474No Hit
CGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTGGAATTCTC3215791.6077912974613136No Hit
CGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGTGGAATTCTCGGGTGCC1688410.8441505522893774No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC1400410.7001598396903399RNA PCR Primer, Index 6 (100% over 50bp)
CGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCATGGAATTCTCG1248950.6244347239603045No Hit
CCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACTGGAATTCTCGGG1115600.5577640242204378No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCCTGGAATTCTCGGGTGCC1101080.5505044924602364No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGGTGGAATTCTC1037210.5185715521348874No Hit
CCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGT906020.45298078273951325No Hit
CCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCTGGAATTCTCGG862830.4313871755271785No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCTGGAATTCTCGGGTGC833020.41648313683767396No Hit
CGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTGGAATTCTC802640.4012941165294838No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG753080.3765157147363995No Hit
CGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCTGGAATTCTCGGGTGC719210.3595818069734502No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGGAATTCTCGGGT663160.33155861446936674No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCA616430.30819512141466876No Hit
TCACCAAGCGTTGGATTGTTCACCCACTAATAGGTGGAATTCTCGGGTGC614520.30724018300819594No Hit
CCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGTGGAATTCTCGGGTG563790.281876818945178No Hit
TCACCAAGCGTTGGATTGTTCACCCACTAATAGTGGAATTCTCGGGTGCC544400.272182444232347No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC508510.2542386016101962No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCGGTGGAATTCTCGG493590.2467790827491627No Hit
GACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTGGAATTCTCGGGTGC476850.23840962257934364No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTG471920.23594478156158927No Hit
CGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGGAATTCTCGGGTGC459550.22976018046835978No Hit
GCGGCGGCGGCGGCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG451000.22548545618807586RNA PCR Primer, Index 1 (100% over 29bp)
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCG445320.22264563935626153No Hit
CCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGG433280.21662602762121844No Hit
CCTCGCCCGTCACGCACCGCCCGTTCGTGGGGAACCTGTGGAATTCTCGG407560.20376685703772107No Hit
CCCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGG396340.1981572188593836No Hit
CCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGGA354120.17704858036656634RNA PCR Primer, Index 1 (100% over 22bp)
GCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTGGAATTCTCG354060.17701858230144155No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGG340200.1700890292576129No Hit
GCGCTAAACCATTCGTAGACGACCTGCTTCTGGTGGAATTCTCGGGTGCC326050.16301448556568102No Hit
GTGCCGCGCGCGGGTCGGGGGGCGGGGTGGAATTCTCGGGTGCCAAGGAA307600.1537900805398052RNA PCR Primer, Index 1 (100% over 23bp)
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCG301350.15066528208930524No Hit
CCCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGG291230.14560560843825573No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGGAATTCTCGGGTGCC287480.14373072936795578No Hit
CCTCTGGCATGTTGGAACAATGTAGGTAAGGTGGAATTCTCGGGTGCCAA274360.13717115246066627No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGG264680.132331464620532Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGTGGAATTCTCGGGTGC249490.1247369544664369No Hit
GCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTGGAATTCTCG247230.12360702734673613No Hit
CCTCGCCCGTCACGCACCTCACGTTCGTGGGGAACCTGTGGAATTCTCGG241330.12065721760946418No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACTGGAATTCTCGGGTG239060.11952229081224261No Hit
CGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCTGGAATTCTCGGGTG235320.11765241141946343No Hit
CCTCTCCCGTCACGCACCGCACGTTCGTGGGGAACCTGTGGAATTCTCGG220000.10999290545759799No Hit
GCCGCCGGTGAAATACCACTACTCTGATCGTTTTGGAATTCTCGGGTGCC218840.1094129428651852No Hit
GCGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTGGAATTCT217160.10857299704169082No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGTGGAATTCTCGGG203920.10195342400415174No Hit
CGCGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTGGAATTC203440.10171343948315334No Hit
CTCTAAGGGCTGGGTCGGTCGGGCTGGGTGGAATTCTCGGGTGCCAAGGA201160.10057351300841096RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)