FastQCFastQC Report
Mon 26 Jun 2023
ERR6548321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6548321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17256430
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length87-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGTGGAATTCTCGG190870811.060850940779755No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCA15237108.829810105566446No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGGTGGAATTCTCG6227863.609008352248988No Hit
CGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTGGAATTCTC5786813.353422463394804No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCCTGGAATTCTCGGGTGCC3225431.8691177723318206No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGTGGAATTCTCG2942161.7049644683170273No Hit
CGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCATGGAATTCTCG2439111.4134499430067515No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCTGGAATTCTCGGGTGC2184711.2660266347094966No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC1854711.074793569701265RNA PCR Primer, Index 8 (100% over 50bp)
CCCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGGTGGAATTCTC1376160.7974766507325096No Hit
CGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTGGAATTCTC1337340.7749806883579048No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAGAGTGGAATTCTCGGGTGCC997700.5781612998748872No Hit
CGCGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTGGAATTC902980.5232716152761608No Hit
CCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACTGGAATTCTCGGG826240.4788012352497011No Hit
TCACCAAGCGTTGGATTGTTCACCCACTAATAGGTGGAATTCTCGGGTGC799270.46317227839130115No Hit
CGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGTGGAATTCTCGGGTGCC740220.4289531496375554No Hit
CCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGGA557430.32302741644708666RNA PCR Primer, Index 1 (100% over 22bp)
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCG557330.32296946703344787No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC505900.2931660835989831No Hit
GGCGGTGGCGGCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC485000.28105465614846176RNA PCR Primer, Index 1 (100% over 31bp)
CCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCTGGAATTCTCGG443960.25727221679107437No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGAGTGGAATTCTCGGGTGC442330.256327641348761No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC426360.2470731199906354No Hit
CCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCTCGCCCGTCAC419500.2430977902150097No Hit
GCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGTGGAATTCTCGGGTG412630.23911666549802016No Hit
GCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTGGAATTCTCG409110.23707684613793237No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGGAATTCTCGGGT407510.23614965551971065No Hit
GCGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTGGAATTCT379500.21991802475946645No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGG375460.2175768684484566No Hit
TCTCCTCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGGAATTC370970.21497493977607188No Hit
TCACCAAGCGTTGGATTGTTCACCCACTAATAGTGGAATTCTCGGGTGCC340490.19731195849894795No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCTGGAATTCTCGG333730.19339457813696112No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCG327470.1897669448431686No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGG323490.18746055818034205Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTGGAATTCTCG313740.18181049035055338No Hit
GCGCTAAACCATTCGTAGACGACCTGCTTCTGGTGGAATTCTCGGGTGCC305200.1768616104257949No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG303500.1758764703939343No Hit
CCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGTGGAATTCTCGGGTGCC294090.1704234305705178No Hit
GTGGCGGCGGCGGCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG290780.16850530497907157RNA PCR Primer, Index 1 (100% over 29bp)
GGCGGTGGCGGCGGCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA273000.1582018992340826RNA PCR Primer, Index 1 (100% over 28bp)
GGCGGTGGCGGCGGCGGCGGCGGCGGCGTGGAATTCTCGGGTGCCAAGGA261740.15167679525834718RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCA258460.14977605449099263No Hit
GCGGCGGCGGCGGCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG249630.14465912126668146RNA PCR Primer, Index 1 (100% over 29bp)
CCTCTGGCATGTTGGAACAATGTAGGTAAGGTGGAATTCTCGGGTGCCAA246300.14272940579250748No Hit
CGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGGAA243140.1408982043215196RNA PCR Primer, Index 1 (100% over 23bp)
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCTGGAATTCT231090.13391529997803717No Hit
CAAGGAGTCTAACACGTGCGCGAGTCGGGGGTGGAATTCTCGGGTGCCAA229860.13320252219027923No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCGGTGGAATTCTCGG224960.13036300092197517No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG217220.12587771630632755No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTG217080.12579658712723316No Hit
CCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGT209710.12152571534204931No Hit
CGGCGGTGGCGGCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT192950.11181339361617669RNA PCR Primer, Index 1 (100% over 30bp)
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGGGTGC191390.11090938276341052No Hit
GCGCTAAACCATTCGTAGACGACCTGCTTCTGTGGAATTCTCGGGTGCCA190780.11055589134021347No Hit
CGCTAAACCATTCGTAGACGACCTGCTTCTGGTGGAATTCTCGGGTGCCA188170.10904341164423928No Hit
GTGGCGGCGGCGGCGGCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTC186740.10821473502920362RNA PCR Primer, Index 1 (100% over 26bp)
CGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGGAATTCTCGGGTGC181800.10535203399544402No Hit
GCGGCGGTGGCGGCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG181110.10495218304133591RNA PCR Primer, Index 1 (100% over 29bp)
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGTGGAATTCTCGGGT180290.10447699784949727No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCTGGAATT180010.10431473949130846No Hit
CCTCGCCCGTCACGCACCGCCCGTTCGTGGGGAACCTGTGGAATTCTCGG178830.10363093641036993No Hit
CGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTC177910.10309780180489245No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGTGGAATTCTCGGGTG176850.10248353802032055No Hit
CCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTGGAATTCTCGGGTG172750.10010761206112737No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)