FastQCFastQC Report
Mon 26 Jun 2023
ERR894498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR894498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36205109
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA7837392.164719349415576No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC4465921.2335054701810177No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG1433930.39605736306442274No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1043320.28816927467336173No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC1003910.27728407059898647No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA976300.26965807505233585No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA903260.24948412667394537No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGGAATTCTCGG832930.23005869144048152No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG770580.21283736502491954No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGGAATTCTCGGGTGCC721840.1993751765807417No Hit
GGAATGCCTAAAGGAAAGATCCAAAAAGATTGGAATTCTCGGGTGCCAAG698180.19284018727854127No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA684790.18914181421191134No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC642410.1774362839233546No Hit
GTTCAGAGTTCTACAGTCCGACGATCGTTCAGAATTCTCGGGTGCCAAGG622100.17182657839809293Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACGATGCCGACTGGCGATGCGGCGGCGTGGAATTCTCGGGTGCCAAGGA611220.1688214776538858RNA PCR Primer, Index 1 (100% over 22bp)
AATAATCCACCTATAACTTCTCTGTTAACCCAATGGAATTCTCGGGTGCC562800.15544767452571404No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC551860.15242600153475577No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCA536600.14821112677771525No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCAATGGAATTCTCGGGTGCCA534690.1476835769228039No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTCTGGAATTCTCGGGTGCCAAG532330.14703173521725899No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGGAATTCTCGGGTGC526220.14534412808976765No Hit
AATAATCCACCTATAACTTCTCTGTTAACCCAACTGGAATTCTCGGGTGC465430.1285536800897354No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGTGGAATTCTCGGGTGCC453140.12515913154687644No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAATGGAATTCTCGGGTGCCA447950.12372563220290264No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCA435560.12030346324879176No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCG431280.1191213096472103No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAATGGAATTCTCGGGTGCCAAGGA426280.1177402890846151RNA PCR Primer, Index 1 (100% over 22bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTC413890.11431812013050424No Hit
TTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAAG410360.11334311961331202No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGTGGAATTCTCGGGTGC409800.11318844531030138No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGA406260.11221068275198398RNA PCR Primer, Index 1 (100% over 22bp)
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG401780.11097328832789871No Hit
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA386160.10665898009035134RNA PCR Primer, Index 1 (100% over 23bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAATGGAATTCTCGGGTGCCA382190.10556244976365076No Hit
GAATGCCTAAAGGAAAGATCCAAAAAGATTGGAATTCTCGGGTGCCAAGG372840.10297994131159777Illumina Small RNA Adapter 2 (100% over 21bp)
ATAATCCACCTATAACTTCTCTGTTAACCCAACTGGAATTCTCGGGTGCC368510.10178397750439033No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA367790.10158511054337663No Hit
AATAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCA367780.10158234850225145No Hit
AATAATCCACCTATAACTTCTCTGTTAACCCAACATGGAATTCTCGGGTG366810.10131443051310798No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)