FastQCFastQC Report
Mon 25 Sept 2023
SRR014368.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR014368.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4485329
Total Bases161.4 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAA47125410.506564847305516No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAA3717998.289224714619596No Hit
AAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAA2718246.060291229472798No Hit
AAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAA1793003.9974771081452443No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1774983.957301682886584No Hit
AGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAA1553333.463135034241635No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAA1487883.3172148575946157No Hit
AGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAA1067172.379245758783804No Hit
CACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAA1010422.252722152600177No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGAAAAAAA807451.8002023931800768No Hit
AGATGGATACGAATAAGGCGTCAAAAAAAAAAAAAA506661.1295938380439874No Hit
AAGAGGTGCACAATCGACCGATCCGAAAAAAAAAAA459631.0247408829987723No Hit
AGTTTCTTTACTTATTCAATGAAGCGAAAAAAAAAA425360.9483362312998668No Hit
AAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAA385170.8587329937224226No Hit
AAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAA372010.8293928940329683No Hit
AAGAGGTGCACAATCGACCGATCCTCAAAAAAAAAA367920.8202742764243157No Hit
AGAGGTGCACAATCGACCGATCCGAAAAAAAAAAAA304210.6782334138699748No Hit
AAGAGGTGCACAATCGACCGATCCTGGAAAAAAAAA298110.6646335196370211No Hit
CTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAA252070.5619877605410886No Hit
CTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAA249920.5571943551966868No Hit
AGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAA235140.5242424803174973No Hit
AAGAGGTGCACAATCGACCGATCCAGAAAAAAAAAA202400.4512489496311196No Hit
AGAGGTGCACAATCGACCGATCCTCAAAAAAAAAAA202080.450535512556604No Hit
AGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAA192010.4280845396179411No Hit
AGAGGTGCACAATCGACCGATCCTGGAAAAAAAAAA191820.4276609363549474No Hit
CACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAA189660.4228452361019671No Hit
AAGAGGTGCACAATCGACCGATCCTTAAAAAAAAAA189160.42173049067303653No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAA187870.41885444746639544No Hit
ATATAGATGGATACGAATAAGGCGTCAAAAAAAAAA166090.37029613658217714No Hit
ATATTAGCATGGAATAATAGAATAAAAAAAAAAAAA158110.35250479953644426No Hit
AGATGGATACGAATAAGGCGTCCAAAAAAAAAAAAA147860.3296525182433663No Hit
CCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAA138290.30831629073363404No Hit
AGAGGTGCACAATCGACCGATCCAGAAAAAAAAAAA131280.29268755982002653No Hit
ATGTATTTATTAGATAAAAAATCAAAAAAAAAAAAA127030.2832122236741162No Hit
ATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAA122300.2726667319164324No Hit
AAGAGGTGCACAATCGACCGATCCGGAAAAAAAAAA115570.25766225844302615No Hit
CAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAA113050.2520439414812157No Hit
AGAGGTGCACAATCGACCGATCCTTAAAAAAAAAAA109780.24475350637600943No Hit
ATATAGATGGATACGAATAAGGCGTCCAAAAAAAAA105840.23596931239603605No Hit
CACGTTCTAGCATTCAAGGTCCCGAAAAAAAAAAAA96500.21514586778361186No Hit
AAGAGGTGCACAATCGACCGATCCTGAGAAAAAAAA94630.2109767198794113No Hit
ATATATTAGCATGGAATAATAGAATAAAAAAAAAAA92600.20645085343795294No Hit
CTTTACTTATTCAATGAAGCGAAAAAAAAAAAAAAA90630.20205875644796625No Hit
CACGTTCTAGCATTCAAGGTCCCTAAAAAAAAAAAA89680.19994074013299803No Hit
AATATAGATGGATACGAATAAGGCGTCAAAAAAAAA82280.18344250778482468No Hit
CACGTTCTAGCATTCAAGGTCCTAAAAAAAAAAAAA81100.1808117085725484No Hit
AAGAGGTGCACAATCGACCGATCCTGTAAAAAAAAA74150.16531674711041264No Hit
CAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAA72630.1619279210064635No Hit
AAATATAGATGGATACGAATAAGGCGTCAAAAAAAA72020.16056793158316815No Hit
ACTTATTCAATGAAGCGGAGAAAAAAAAAAAAAAAA71170.15867286435398606No Hit
AGAGGTGCACAATCGACCGATCCGGAAAAAAAAAAA69550.15506108916425082No Hit
AATATATTAGCATGGAATAATAGAATAAAAAAAAAA68120.15187291723750923No Hit
AGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAA64750.14435953304651675No Hit
GTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAAA64710.14427035341220232No Hit
AGAGGTGCACAATCGACCGATCCTGAGAAAAAAAAA64110.14293265889748555No Hit
CACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAA59930.1336133871116255No Hit
AAGAGGTGCACAATCGACCGATCCTGCAAAAAAAAA57060.12721474834956364No Hit
AGTTTCTTTACTTATTCAATGAAGCGAAGAAAAAAA56940.1269472094466203No Hit
ATAGATGGATACGAATAAGGCGTCCAAAAAAAAAAA56810.12665737563509832No Hit
AGATTAAGCCTTTGTTGTCTGATTTGTAAAAAAAAA56240.1253865658461174No Hit
AGATGGATACGAATAAGGCGCCAAAAAAAAAAAAAA55850.12451706441155154No Hit
AATATAGATGGATACGAATAAGGCGTCCAAAAAAAA55090.12282265135957697No Hit
AAGAGGTGCACAATCGACCGATCCTGAAGAAAAAAA53690.11970136415857122No Hit
AAATCAGTTATCGTTTATTTGATAGTTAAAAAAAAA53000.11816301546664693No Hit
CACGTTCTAGCATTCAAGGTCACAAAAAAAAAAAAA51970.11586663988304982No Hit
AAGCCTTTGTTGTCTGATTTGTAAAAAAAAAAAAAA49240.10978012984108859No Hit
AGAGGTGCACAATCGACCGATCCTGCAAAAAAAAAA48890.10899980804083714No Hit
CAAGAGGTGCACAATCGACCGATCCCGAAAAAAAAA47590.1061014699256175No Hit
AATTAGAGTGTTCAAAGCAGGCGTAAAAAAAAAAAA47580.10607917501703888No Hit
AGAGGTGCACAATCGACCGATCCTGTAAAAAAAAAA47480.10585622593125275No Hit
AAATATAGATGGATACGAATAAGGCGTCCAAAAAAA46380.10340378598760536No Hit
AGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAA45530.1015087187584233No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)