FastQCFastQC Report
Mon 25 Sept 2023
SRR014373.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR014373.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5072716
Total Bases177.5 Mbp
Sequences flagged as poor quality0
Sequence length35
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAA1064022.097535127138992No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAA838001.651974997220424No Hit
AGAGGTGCACAATCGACCGATCCTAAAAAAAAAAA475890.9381364933499136No Hit
TTTTCTTTACTTATTCAATGAAGCGAAAAAAAAAA417210.8224588169335717No Hit
GTTTCTTTACTTATTCAATGAAGCGAAAAAAAAAA406850.8020358324810615No Hit
AAGAGGTGCACAATCGACCGATCCTAAAAAAAAAA356110.7020105205968558No Hit
GTTTCTTTACTTATTCAATGAAGCAAAAAAAAAAA336570.663490721735654No Hit
GTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAA321240.6332702244714666No Hit
AGTTTCTTTACTTATTCAATGAAGCGAAAAAAAAA317140.6251877692344693No Hit
AGTTTCTTTACTTATTCAATGAAGCAAAAAAAAAA316320.6235712781870698No Hit
AGAGGTGCACAATCGACCGATCCAAAAAAAAAAAA268500.5293022514960427No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAAAAAAAA242240.4775351113683478No Hit
TTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAA234570.4624150060835261No Hit
TTTCTTTACTTATTCAATGAAGCGAAAAAAAAAAA220330.43434325911405247No Hit
AAGAGGTGCACAATCGACCGATCCAAAAAAAAAAA210540.41504393307253945No Hit
AAGAGGTGCACAATCGACCGATCCCGAAAAAAAAA191120.37676069387681077No Hit
AGAGGTGCACAATCGACCGATCCCGAAAAAAAAAA180470.35576602356607384No Hit
TTTTCTTTACTTATTCAATGAAGCAAAAAAAAAAA174300.3436029140996657No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGAAAAAA173800.34261724882686123No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGAAAAAAA158500.312455891479042No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAACA144360.2845812775641293No Hit
TTTCTTTACTTATTCAATGAAGCGGAAAAAAAAAA129050.2544002069108541No Hit
TATAGATGGATACGAATAAGGCGTCAAAAAAAAAA117710.2320453185236469No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAACA117070.23078366697445707No Hit
AGTTTCTTTACTTATTAAATGAAGCGAAAAAAAAA116890.23042882747624743No Hit
AGATGGATACGAATAAGGCGTCAAAAAAAAAAAAA114390.2255005011122247No Hit
TTTCTTTACTTATTCAATGAAGCAAAAAAAAAAAA101420.19993234393567472No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAACAA100280.19768502711368033No Hit
AAGAGGTGCACAATCGACCGATCCCAAAAAAAAAA100140.19740904083729505No Hit
AGAGGTGCACAATCGACCGATCCCAAAAAAAAAAA95660.18857747999296628No Hit
AGAGGTGCACAATCGACCGATCCTGAAACAAAAAA94760.1868032825019181No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAACAAA89700.17682834994113605No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAACAAA89330.1760989576392607No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAC80650.15898780850337374No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAACAA78280.15431575511028017No Hit
AAGAGGTGCACAATCGACCGATCCTGAACAAAAAA76880.15155589234642744No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAACAAAA75810.1494465686626257No Hit
TCTTTACTTATTCAATGAAGCGAAAAAAAAAAAAA73650.14518849468411005No Hit
TGTTTCTTTACTTATTCAATGAAGCGAAAAAAAAA70640.13925478974182667No Hit
AGAGGTGCACAATCGACCGATCCTGCAAAAAAAAA68850.1357261080651864No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAC66120.13034437567567356No Hit
TCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAA63900.12596802186442135No Hit
AGAGGTGCACAATCGACCGATCCTAAAAAAAAACA63320.12482465014796806No Hit
AAGAGGTGCACAATCGACCGATCCTGCAAAAAAAA62650.12350385868240997No Hit
AGAGGTGCACAATCGACCGATCCTGACAAAAAAAA62340.12289274621327115No Hit
GTTTCTTTACTTATTAAATGAAGCGGAAAAAAAAA61840.1219070809404666No Hit
AGAGGTGCACAATCGACCGATCCTGAAAACAAAAA61240.12072428261310116No Hit
CTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAA61000.12025116328215496No Hit
AGAGGTGCACAATCGACCGATCCAGAAAAAAAAAA60030.11833897265291413No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAACAAAA59880.11804327307107278No Hit
TGTTTCTTTACTTATTCAATGAAGCAAAAAAAAAA59500.11729416746374133No Hit
TGAGGTGCACAATCGACCGATCCTGAAAAAAAAAA58880.11607194252546368No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGAAAAAAA58680.11567767641634186No Hit
GTTTTCTTTACTTATTCAATGAAGCAAAAAAAAAA56760.1118927217687724No Hit
AGAGGTGCACAATCGACCGATCCTGAACAAAAAAA55360.10913285900491965No Hit
TGTTTCTTTACTTATTCAATGAAGCGGAAAAAAAA55120.10865973967397347No Hit
TTCTTTACTTATTCAATGAAGCGAAAAAAAAAAAA54340.10712210184839836No Hit
AGTTTTCTTTACTTATTCAATGAAGCAAAAAAAAA52920.10432281247363345No Hit
AAGAGGTGCACAATCGACCGATCCAGAAAAAAAAA51860.1022332020952878No Hit
AAGAGGTGCACAATCGACCGATCCTGAAACAAAAA51480.10148409648795635No Hit
AAGAGGTGCACAATCGACCGATCCTGACAAAAAAA51450.10142495657158809No Hit
AGAGGTGCACAATCGACCGATCCGAAAAAAAAAAA50840.10022244493876652No Hit

[OK]Adapter Content

Adapter graph