FastQCFastQC Report
Mon 25 Sept 2023
SRR057512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR057512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18618732
Total Bases670.2 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTC10452745.6140987474334985No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACT9895245.314669119250441No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTCG4197812.254616479790353No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATC2297881.2341764197475962No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCATCGT1665130.8943305054286189No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTC1443440.7752622466449379No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATCG1332410.7156287549549561No Hit
CTAAACCATTCGTAGACGACCTGCTTTCGTATGCCG946870.508557725628147No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAT748640.40208968043581056No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTC501960.26959945500048016No Hit
TTTGGTGACTCTAGATAACCTCGGGCTGATCGCACT494070.2653617872581226No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT486710.2614087790726028No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN460630.24740138050217383No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATATC423360.227383905627945No Hit
TACTTGGATAACTGTGGTAATTCTAGAGTCGTATGC417330.2241452318020368No Hit
TTGGTGACTCTAGATAACCTCGGGCTGATCGCACTC399840.21475146642639253No Hit
CATTGATCATCGACACTTCGAACGCATCGTATGCCG398730.21415529263754374No Hit
TACTTGGATAACTGTGGTAATTCTAGTCGTATGCCG372220.1999169438606238No Hit
ATTGATCATCGACACTTCGAACGCATCGTATGCCGT355970.19118917442927907No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTCT348130.1869783613620949No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCG343530.184507731246145No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATG341450.1833905767589329No Hit
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCG324220.17413645569419012No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGTCGTATGC276860.14869970736997556No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCC254280.1365721360616824No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC246540.13241503234484497No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACTCGT240520.1291817294539714No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTCG230180.12362818262811884No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATTCGT225820.12128645495300111No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTCGTATG215450.11571679532204449No Hit
TTGGTGACTCTATATAACCTCGGGCCGATCGCACTC205100.11015787756115723No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTCG203200.10913739990456922No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACTCG197130.1058772423385223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)