FastQCFastQC Report
Mon 25 Sept 2023
SRR057516.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR057516.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5901290
Total Bases212.4 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACT5758439.757917336717904No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTC4189027.098481857356612No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2247643.80872656656426No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTCG956021.620018673883168No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATC902501.529326638751866No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTC668091.13210840341688No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTC567410.9615016377775029No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATCG558180.9458609897158079No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAT556890.9436750269856252No Hit
CTAAACCATTCGTAGACGACCTGCTTTCGTATGCCG342690.5807035410901684No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCATCGT297590.5042795727713771No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCG283520.4804373281096167No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT267980.4541041026622993No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCT230820.39113481967502023No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATG202370.34292502147835474No Hit
TTTGGTGACTCTAGATAACCTCGGGCTGATCGCACT190620.32301412064141904No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCG182820.30979667157519797No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTCG145300.24621735247717025No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATTCGT141710.24013393681720438No Hit
CTGGATACCGCAGCTAGGAATAATGGAATTCGTATG133810.2267470332757753No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATATC131030.22203619886499393No Hit
CATTGATCATCGACACTTCGAACGCATCGTATGCCG125970.213461802419471No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCC114940.19477097380403266No Hit
TTGGTGACTCTAGATAACCTCGGGCTGATCGCACTC113530.1923816657036004No Hit
TACTTGGATAACTGTGGTAATTCTAGTCGTATGCCG111990.18977206678539776No Hit
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCG108390.1836717056779111No Hit
TACTTGGATAACTGTGGTAATTCTAGAGTCGTATGC104560.17718159927744612No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTCG98770.16737018516290506No Hit
ATTGATCATCGACACTTCGAACGCATCGTATGCCGT78260.13261507229775185No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTCGTATG75820.12848038310267756No Hit
TGGATACCGCAGCTAGGAATAATGGAATTCGTATGC73440.12444736659272804No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAT73040.12376954869189617No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCGT72690.1231764580286683No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGTCGTATGC72310.12253253102287806No Hit
CTGGATACCGCAGCTAGGAATAATGGAATATCGTAT71370.1209396589559232No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACTCGT68640.1163135517827458No Hit
CTAAACCATTCGTAGACGACCTGCTTCTCGTATGCC64020.10848475502813791No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTAT62560.10601071969010166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)