FastQCFastQC Report
Mon 25 Sept 2023
SRR057517.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR057517.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14520158
Total Bases522.7 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACT10087426.947183357095701No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTC7013854.83042264416131No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4183312.8810361429951383No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTCG1987641.3688831760646132No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATC1549111.0668685561135078No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTC1389170.9567182395673656No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTC1223950.8429315989536753No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAT1052450.7248199365323711No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATCG891120.6137123301275371No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCATCGT641140.44155166906586No Hit
CTAAACCATTCGTAGACGACCTGCTTTCGTATGCCG628550.4328809645184302No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT597020.4111663247741519No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCG567520.3908497414422074No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCT429540.29582322726791266No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCG426570.2937777949799169No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATG417180.28731092320069795No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN373670.25734568453043005No Hit
TTTGGTGACTCTAGATAACCTCGGGCTGATCGCACT339040.23349608179194745No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATTCGT334910.23065176012547522No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATATC310310.21370979571985377No Hit
CATTGATCATCGACACTTCGAACGCATCGTATGCCG302740.20849635382755477No Hit
TACTTGGATAACTGTGGTAATTCTAGTCGTATGCCG266860.18378587891398976No Hit
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCG265310.1827183974168876No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTCG256840.1768851275585293No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCC255020.17563169767160935No Hit
CTGGATACCGCAGCTAGGAATAATGGAATTCGTATG253620.1746675208355171No Hit
TACTTGGATAACTGTGGTAATTCTAGAGTCGTATGC247760.17063175207873083No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTCG229750.1582283057801437No Hit
ATTGATCATCGACACTTCGAACGCATCGTATGCCGT191010.1315481553299902No Hit
TTGGTGACTCTAGATAACCTCGGGCTGATCGCACTC190770.13138286787237438No Hit
TGGATACCGCAGCTAGGAATAATGGAATTCGTATGC188530.12984018493462673No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAT182340.12557714592361874No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTCGTATG163790.11280180284539602No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGTCGTATGC160850.11077703148960226No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCTCACT155620.10717514230905753No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCGT153990.10605256499275008No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACTCGT149910.10324267821328116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)