FastQCFastQC Report
Mon 25 Sept 2023
SRR057521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR057521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5910808
Total Bases212.7 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACT77001813.027288316588868No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTC4333277.3310958501781815No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2788594.717781393000754No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTC1053481.782294400359477No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATC839191.4197551333083396No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTCG651751.1026411279134765No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTC525370.8888294121548188No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAT456760.7727539111404058No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATCG433790.7338928958612765No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCT395100.6684365318582501No Hit
CTAAACCATTCGTAGACGACCTGCTTTCGTATGCCG343220.5806651138050839No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT292820.49539758354526153No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCG270010.4568072588383856No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATG269150.45535229701252355No Hit
TTTGGTGACTCTAGATAACCTCGGGCTGATCGCACT264570.44760377938176976No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCATCGT154980.2621976555489537No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCG126320.21371020679406266No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTCG125410.21217065416437145No Hit
TTGGTGACTCTAGATAACCTCGGGCTGATCGCACTC123080.20822872270593124No Hit
CTGGATACCGCAGCTAGGAATAATGGAATTCGTATG121530.2056064077872264No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCC115130.19477878489709022No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATTCGT103410.17495070047952835No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAT103400.17493378231876253No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTCG101210.17122870511104404No Hit
TACTTGGATAACTGTGGTAATTCTAGTCGTATGCCG90970.15390450848682616No Hit
CTGGATACCGCAGCTAGGAATAATGGAATATCGTAT86300.14600372740917994No Hit
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCG82290.13921954494207897No Hit
TACTTGGATAACTGTGGTAATTCTAGAGTCGTATGC77530.13116650041754022No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTAT66980.11331784080958135No Hit
CTTTGGTGACTCTAGATAACCTCGGGCTGATCGCAC65830.11137225232151003No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATATC62540.1058061774295494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)