FastQCFastQC Report
Mon 25 Sept 2023
SRR057522.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR057522.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14178182
Total Bases510.4 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACT13918969.817168378851393No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTC7527095.308924656207686No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC5368433.786402234080505No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTC2045811.4429282964487267No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATC1511841.0663144259256936No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTCG1511091.0657854441422743No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTC1444741.0189881890358017No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAT893800.6304052240266065No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCT765050.5395966845396681No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATCG753550.5314856305272425No Hit
CTAAACCATTCGTAGACGACCTGCTTTCGTATGCCG696720.491402917524969No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT696580.49130417425873074No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATG597530.42144331339518704No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCG595390.41993395203983136No Hit
TTTGGTGACTCTAGATAACCTCGGGCTGATCGCACT485460.3423993287714885No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN400470.2824551130744407No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCATCGT365430.25774108415310226No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCG325730.2297403150841201No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAT284390.20058283918206157No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCC270580.19084252127670528No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATTCGT268940.18968581444362895No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTCG251600.17745575561098031No Hit
CTGGATACCGCAGCTAGGAATAATGGAATTCGTATG251030.17705372945558184No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTCG244180.17222236250035441No Hit
TTGGTGACTCTAGATAACCTCGGGCTGATCGCACTC218920.15440625603480052No Hit
TACTTGGATAACTGTGGTAATTCTAGTCGTATGCCG218380.1540253891507388No Hit
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCG217240.15322133683994182No Hit
TACTTGGATAACTGTGGTAATTCTAGAGTCGTATGC191710.13521479693235705No Hit
CTGGATACCGCAGCTAGGAATAATGGAATATCGTAT178780.12609515098621246No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTAT158460.11176327120077877No Hit
TGGATACCGCAGCTAGGAATAATGGAATTCGTATGC155290.10952744152952754No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATATC148720.1048935611067766No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTC144810.1021358027425519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)