FastQCFastQC Report
Mon 25 Sept 2023
SRR057526.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR057526.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20366374
Total Bases733.1 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACT17851828.765340359555413No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC15884767.799503239997459No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTC9668954.74750684633406No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTCG2492361.2237622661746268No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTC1705070.8371986098261772No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAT1088350.5343857478017442No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATC951640.4672603969660971No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTC889210.4366069286560288No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCT832130.4085803393377731No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN763560.3749120977548581No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCATCGT759670.37300208667482976No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT717690.35238967918393327No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATCG675190.33152194887514097No Hit
TTTGGTGACTCTAGATAACCTCGGGCTGATCGCACT568930.2793477130489698No Hit
CTAAACCATTCGTAGACGACCTGCTTTCGTATGCCG565220.27752608294436704No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATG503090.2470199162600078No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCG376960.18508940275770247No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATATC368820.18109261864679496No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATAT343140.16848359948609407No Hit
TACTTGGATAACTGTGGTAATTCTAGAGTCGTATGC330730.16239022223592672No Hit
CTTTGGTGACTCTAGATAACCTCGGGCTGATCGCAC324390.15927724787927394No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCC280760.13785468144697727No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAT276970.135993770908852No Hit
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCG246710.12113594692899188No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTCG245760.12066949178091298No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTCGTATG238360.11703605168008797No Hit
TTGGTGACTCTAGATAACCTCGGGCTGATCGCACTC237820.11677090875381155No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTCG233860.11482652729445114No Hit
TACTTGGATAACTGTGGTAATTCTAGTCGTATGCCG224560.11026017689746835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)