FastQCFastQC Report
Mon 25 Sept 2023
SRR057529.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR057529.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20233514
Total Bases728.4 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACT7550353.731605889120397No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTC6587343.2556579148831983No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4948502.4456948012095183No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTCG2572311.2713115477617976No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTC1568670.7752830279505577No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTC862990.4265151372124486No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT841710.41599793293443743No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN829040.4097360448610162No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCATCGT786130.38852865597147385No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATC570990.2822001161043999No Hit
CTAAACCATTCGTAGACGACCTGCTTTCGTATGCCG568800.2811177534460895No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATCG526950.2604342478523503No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCT523500.2587291559933682No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATG512790.25343595778765865No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAT482070.23825322679985297No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATATC406640.20097349377868814No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTCG357310.1765931513428661No Hit
TACTTGGATAACTGTGGTAATTCTAGAGTCGTATGC321570.1589293881428604No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTA309330.1528800187649066No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCG308670.15255382727884043No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTCGTATG293750.14517992277564834No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCC287530.14210581513423717No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTCG283710.14021785835124834No Hit
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCG265590.1312624193701598No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATAT256190.12661666184133907No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTAT250460.12378472666685579No Hit
TTTGGTGACTCTAGATAACCTCGGGCTGATCGCACT247000.12207469251263028No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACTCGT233850.11557557426752467No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCG230480.11391002077048998No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAT218000.10774203630669393No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTA216980.10723792219186445No Hit
TACTTGGATAACTGTGGTAATTCTAGTCGTATGCCG207900.10275031811083334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)