FastQCFastQC Report
Mon 25 Sept 2023
SRR057532.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR057532.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18817795
Total Bases677.4 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACT17099569.086909491786896No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTC11666536.199732752960695No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC5671253.013769679178671No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTCG2917211.5502400786064467No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTC2412621.2820949532078547No Hit
CTAAACCATTCGTAGACGACCTGCTTTCGTATGCCG1021400.5427841040887097No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATC958200.5091988726628173No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAT942700.5009619883732392No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCATCGT880330.4678178288157565No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATCG745270.3960453389996012No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN742700.3946796104432002No Hit
TTTGGTGACTCTAGATAACCTCGGGCTGATCGCACT674840.3586179996115379No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCT641250.34076787423818783No Hit
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCG572100.3040207420688768No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTCGTATG514700.2735176996029556No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT501720.2666199732752961No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATG405740.21561506010667034No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTCG391590.20809558186812002No Hit
TTGGTGACTCTAGATAACCTCGGGCTGATCGCACTC370790.19704221456339596No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGTCGTATGC360600.19162712740786048No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTC325500.17297457008113865No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTCGTA310160.16482271169390464No Hit
TACTTGGATAACTGTGGTAATTCTAGAGTCGTATGC302470.1607361542624946No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACTCGT276270.14681316275365952No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACTCG258870.13756659587374612No Hit
AGCGTGTACTCCGAAGAGGATCCAAACTCGTATGCC255030.13552597421748935No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCC241250.12820311837810966No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTCGTAT237730.12633254852654097No Hit
CTAAACCATTCGTAGACGACCTGCTTCTCGTATGCC234750.1247489410953834No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTCG226760.12050296009707831No Hit
TACTTGGATAACTGTGGTAATTCTAGTCGTATGCCG208170.11062401306848119No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATATC207490.11026265298351905No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGTCGTATGC192350.1022170769742151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)