FastQCFastQC Report
Mon 25 Sept 2023
SRR065774.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR065774.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6111972
Total Bases220 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTC2396863.9215821014886845No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCGT2044393.344894250169994No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCG1398152.2875595634273194No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTC1020711.6700174673575077No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGTC836231.3681836238778582No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCG754951.2351987214601114No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCTCGT579750.9485481936108345No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATCG533580.873007926083431No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATC384500.6290931961075737No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACT370690.6064981973084955No Hit
CTAAACCATTCGTAGACGACCTGCTTTCGTATGCCG270400.4424104037125825No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTCG258030.42217143664925166No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACATC252280.41276367103776No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTC243380.3982020860043207No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCGT214610.35113053528386584No Hit
CTAGACCATTCGTAGACGACCTGCTTTCGTATGCCG211550.34612396784540245No Hit
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCG196100.32084571068061174No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTCG194940.3189477962268152No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATTCGT185300.30317547266250566No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTCG169440.2772264009062869No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTCTCGT166600.27257978276078487No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT151290.24753058423696966No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATTCG132230.21634588640131205No Hit
TTGGTGACTCTAGATAACCTCGGGCCGTCGTATGCC123830.20260236794278508No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCTCG109680.17945108387276643No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGATCGT109150.17858393330335937No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTCGTAT101910.166738329298629No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT95370.15603801849877585No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACTCG93550.153060256166095No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTTCGTA91550.14978798986644573No Hit
AGCGTGTACTCCGAAGAGGATCCAAACTCGTATGCC90330.14779190742365966No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTCG87590.14330890259314016No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATATC85550.13997119096749788No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGTATGC81570.1334593810311958No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGTCGTA75870.12413342207719538No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGTCGTATGC72240.11819425874333195No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATG70740.11574005901859499No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATCG69170.1131713299733703No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTTCGTATGCC66240.1083774598443841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)