FastQCFastQC Report
Mon 25 Sept 2023
SRR065775.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR065775.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12962679
Total Bases466.6 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTC4844073.736935860249259No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCGT3926873.029366074713414No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCG2491201.9218249560912524No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTC1853401.4297970350110498No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGTC1618691.2487310686317235No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCG1195480.9222476310645353No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCTCGT958030.7390679040960592No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATCG875690.6755470840556956No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATC711380.5487908787990508No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACT691470.5334313994815424No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACATC539380.41610225787431754No Hit
CTAAACCATTCGTAGACGACCTGCTTTCGTATGCCG457860.3532140231197579No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTC434910.33550934957195194No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTCG423270.3265297242954176No Hit
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCG382620.295170465919892No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN381080.29398243989533335No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATTCGT379450.29272498377843037No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTCG372760.28756401358083467No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCGT361080.2785535304854807No Hit
CTAGACCATTCGTAGACGACCTGCTTTCGTATGCCG342910.26453636628662947No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTCTCGT331590.25580360356065285No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCT317750.24512679824903477No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT294920.22751469815768793No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTCG270670.20880714549824153No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATTCG238170.1837351677072309No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCTT215980.1666167927169993No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCTCG211740.16334586392211053No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTCGTAT208570.1609003817806489No Hit
TTGGTGACTCTAGATAACCTCGGGCCGTCGTATGCC204880.15805374799453106No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGATCGT204490.15775288426103895No Hit
AGCGTGTACTCCGAAGAGGATCCAAACTCGTATGCC190940.14729979813586375No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTTCGTA190650.14707607894942087No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATTGAATTC189160.14592662519838684No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT173520.13386121803988205No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACTCG172720.13324406166348793No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCT168200.12975712813686122No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTCG159140.12276783217419794No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGTCGTA147130.11350277207358139No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATATC146410.1129473313348267No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGTATGC136260.10511715980932644No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTTCGTATGCC134820.10400627833181705No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATG133250.10279510894314363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)