FastQCFastQC Report
Mon 25 Sept 2023
SRR065779.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR065779.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5883823
Total Bases211.8 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTC2931974.98310367256119No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTC1138611.9351533858173504No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCG1107531.8823305867630622No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCG808991.3749393888973207No Hit
CTAAACCATTCGTAGACGACCTGCTTTCGTATGCCG731031.2424405016942217No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATCG728641.2383785168248602No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCGT710311.207225302324696No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACT539260.9165129542476039No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATC519880.8835751857253354No Hit
CTAGACCATTCGTAGACGACCTGCTTTCGTATGCCG505250.8587103996840149No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTC421000.7155211841008814No Hit
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCG396560.6739835647673289No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGTC354830.6030602892031253No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCTCGT324620.5517161206242948No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAT295560.5023264635934834No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTCG272060.4623864450035291No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACATC255910.4349383045683054No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCGT220560.3748583191574594No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT177910.3023714343548404No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTCG176420.2998390672187114No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATTCGT174790.2970687595463018No Hit
TTGGTGACTCTAGATAACCTCGGGCCGTCGTATGCC151330.25719672396671345No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT151290.2571287409563476No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTCG146220.24851189439247237No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATG141800.24099977174704268No Hit
AGCGTGTACTCCGAAGAGGATCCAAACTCGTATGCC118040.20061786358971032No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTCTCGT113500.192901791913183No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATATC109940.18685130399061972No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTCG95640.1625473777848178No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88940.15116022354853298No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGTATGC88880.15105824903298418No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTCGTA85350.14505874836819532No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCTCG79530.13516722035995984No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTCGTAT77010.13088429070690943No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGTCGTA72410.12306624451483329No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGTCGTATGC70910.1205168816261128No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTTCGTA68910.11711773110781884No Hit
GCCGCCTGGATACCGCAGCTCGGAATAATGGAATTC63020.10710723283144309No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGATCGT62440.10612147918113785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)