FastQCFastQC Report
Mon 25 Sept 2023
SRR065780.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR065780.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15253899
Total Bases549.1 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTC7679125.034201419584592No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCG2686321.7610710546857562No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTC2085171.366975092728751No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCGT1912371.253692580500238No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCG1558171.0214896532355433No Hit
CTAAACCATTCGTAGACGACCTGCTTTCGTATGCCG1427840.9360492028956006No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATCG1235900.8102190790695546No Hit
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCG1084360.7108739870376748No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACT1039090.6811963288861425No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATC977550.6408525453066131No Hit
CTAGACCATTCGTAGACGACCTGCTTTCGTATGCCG954020.6254269809967931No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGTC947000.6208248789375097No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTC827650.5425825882287538No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAT769280.504316961846935No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACATC736100.4825651461308351No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCTCGT572010.37499264942032196No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTCG499790.3276473772377803No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATTCGT471520.30911441068280315No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTCG459430.3011885682473707No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCGT451500.2959898974026247No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT440810.28898185309867336No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN436230.2859793420685426No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTAT354430.23235370838629518No Hit
AGCGTGTACTCCGAAGAGGATCCAAACTCGTATGCC342890.22478842950251604No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATG324620.2128111638866889No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTCTCGT313030.205213106498214No Hit
TTGGTGACTCTAGATAACCTCGGGCCGTCGTATGCC273060.17900996984443124No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTCG256970.16846184703333883No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTCGTAT242220.15879218814809248No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATATC226420.14843418066423542No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTCGTA222400.1457987888866971No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTCG218760.14341251374484648No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCT217870.1428290563612621No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCTCG208100.13642413654371252No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTTCGTA196460.12879330065054187No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGTCGTA191940.12583012382604605No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGATCGT167910.11007677446926847No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGTATGC156940.10288517053902088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)