FastQCFastQC Report
Mon 26 Jun 2023
SRR074359.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074359.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2785131
Total Bases114.1 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA1295124.650122382035172No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA526911.8918679229091917No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA487911.7518386029238842No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA402021.4434509543716256No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA331691.1909314139981209No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAA328731.1803035476607744No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA320211.149712526987061No Hit
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT287421.0319801833378752Illumina PCR Primer Index 2 (95% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA269290.9668845020216285No Hit
TCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG258190.9270300032565792Illumina Paired End PCR Primer 2 (95% over 22bp)
ACGAGACTCTGGCATGCTAACTAGTTACAAAAAAAAAAAAA219660.7886882161018637No Hit
ACGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA213640.767073433888747No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT208820.7497672461367167No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA198100.7112771356176782No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT193510.6947967618040228Illumina PCR Primer Index 1 (95% over 21bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA191510.6876157710355456No Hit
ACAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG184310.6617642042690273Illumina Paired End PCR Primer 2 (95% over 22bp)
TTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT170910.6136515661202292Illumina Paired End PCR Primer 2 (95% over 21bp)
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG164400.5902774411688355Illumina Paired End PCR Primer 2 (95% over 22bp)
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT159110.571283720586213Illumina Paired End PCR Primer 2 (95% over 21bp)
TCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA156360.5614098582795567Illumina PCR Primer Index 2 (95% over 22bp)
TTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG152550.5477300708656074Illumina Paired End PCR Primer 2 (95% over 22bp)
TCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA147610.5299930236674685No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA147250.5287004453291425No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT146500.5260075737909635Illumina Paired End PCR Primer 2 (95% over 21bp)
ATAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG139760.501807634901195Illumina Paired End PCR Primer 2 (95% over 22bp)
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT133670.47994151801118146No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA127880.4591525497364397No Hit
GCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA117160.42066243921740126Illumina PCR Primer Index 2 (95% over 22bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA112560.4041461604499035No Hit
GTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG112350.40339215641921333Illumina Paired End PCR Primer 2 (95% over 22bp)
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT110200.39567259134310023No Hit
ACAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA109690.3938414386971385Illumina Paired End PCR Primer 2 (95% over 22bp)
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT107930.3875221668208785No Hit
CCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG97250.34917567611720957Illumina PCR Primer Index 2 (95% over 22bp)
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT95820.3440412677177483Illumina PCR Primer Index 2 (95% over 21bp)
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT92630.33258758744202693No Hit
CCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT90530.3250475471351258Illumina PCR Primer Index 2 (95% over 21bp)
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT89930.32289324990458257No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAAAAAAAA87670.3147787303362032No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA85970.3086748881829975No Hit
TTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA85620.30741821479851394Illumina PCR Primer Index 1 (95% over 22bp)
GTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA85520.3070591652600901Illumina PCR Primer Index 1 (95% over 22bp)
ATAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA76840.2758936653248985Illumina Paired End PCR Primer 2 (95% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA76690.27535509101726274No Hit
CTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG76420.2743856572635183Illumina PCR Primer Index 1 (95% over 22bp)
CTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT75050.2694666785871114Illumina Paired End PCR Primer 2 (95% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA73870.2652298940337097No Hit
TCAACTTCTTAGAGGGACAAGTGGCGCAAAAAAAAAAAAAA73450.2637218859723295No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAA71640.2572230893268575No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA71430.2564690852961674No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA69230.24856999545084232No Hit
TCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAAA65960.23682907554438193No Hit
CCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA64820.23273591080634984Illumina PCR Primer Index 1 (95% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAA63400.227637407360731No Hit
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTT60130.21589648745427054No Hit
ACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAA59010.21187513262392327No Hit
CCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT58300.20932588090111381No Hit
TCAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA57670.20706386880904346Illumina Paired End PCR Primer 2 (95% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA55920.2007805018866258No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAA53360.19158883370297483No Hit
TCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC52630.1889677720724806No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT52220.18749566896494274No Hit
CTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA52030.18681347484193742Illumina PCR Primer Index 2 (95% over 22bp)
TCAACTTCTTAGAGGGACAAGTGGCGTAAAAAAAAAAAAAA50460.18117639708868272No Hit
GGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAA50030.1796324840734601No Hit
CGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG49690.17841171564281896Illumina PCR Primer Index 1 (95% over 22bp)
TCAACTTCTTAGAGGGACAAGTGGCAAAAAAAAAAAAAAAA48400.17377997659715108No Hit
TCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTTG48000.1723437784434556Illumina Single End Adapter 1 (95% over 21bp)
AACGAGACTCTGGCATGCTAACTAGTTACAAAAAAAAAAAA47830.17173339422813505No Hit
ACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAA47480.17047672084365154No Hit
GCAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA47340.1699740514898581Illumina PCR Primer Index 2 (95% over 22bp)
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT46480.16688622545941287No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA45670.16397792419817955No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAA45330.1627571557675384No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAAA44760.16071057339852238No Hit
CTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT43970.15787408204497383No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAA42910.15406815693768083No Hit
TGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG42330.15198566961482243Illumina Paired End PCR Primer 2 (95% over 22bp)
CGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA42320.15194976466098006Illumina Paired End PCR Primer 2 (95% over 22bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA41720.14979546743043687No Hit
ACAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA40530.14552277792319285Illumina Paired End PCR Primer 2 (95% over 22bp)
TTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTT39840.14304533610806816No Hit
TTAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA39400.14146551813900315Illumina Paired End PCR Primer 2 (95% over 22bp)
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTT37970.13633110973954188No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAA37850.13590025029343325No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTT37420.1343563372782106No Hit
ACAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC36910.1325251846322489No Hit
GTAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA36070.12950916850948843Illumina PCR Primer Index 1 (95% over 22bp)
TCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAA35980.12918602392490694No Hit
CGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT35330.12685220192515181Illumina Paired End PCR Primer 2 (95% over 21bp)
ATAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA34360.12336942140244032Illumina Paired End PCR Primer 2 (95% over 22bp)
TCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGC33600.12064064491041893No Hit
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTT32770.11766053374150086No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTT32740.11755281887997368No Hit
TGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA32710.11744510401844653Illumina Paired End PCR Primer 2 (95% over 22bp)
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA32210.1156498563263272No Hit
TGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT32080.11518309192637616Illumina Paired End PCR Primer 2 (95% over 21bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA31550.11328012937272969No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAAAA31470.11299288974199058No Hit
CGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAAAA31390.1127056501112515No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT31290.11234660057282764No Hit
TCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGT31070.11155669158829512No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT30190.10839705565016511No Hit
TTAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAA30150.10825343583479557Illumina Paired End PCR Primer 2 (95% over 22bp)
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT29190.10480656026592644No Hit
ACAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTTG28880.10369350669681247Illumina Single End Adapter 1 (95% over 21bp)
ACGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA28530.10243683331232893No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAA28350.10179054414316599No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)