FastQCFastQC Report
Mon 26 Jun 2023
SRR074360.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074360.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5086078
Total Bases208.5 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT555071.091351725238976Illumina PCR Primer Index 2 (95% over 21bp)
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT550601.0825630279362606Illumina PCR Primer Index 1 (95% over 21bp)
GGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAA530831.0436922123490833No Hit
TCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG522861.0280219847198568Illumina Paired End PCR Primer 2 (95% over 22bp)
ACAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG491250.9658719351138539Illumina Paired End PCR Primer 2 (95% over 22bp)
ACGAGACTCTGGCATGCTAACTAGTTACAAAAAAAAAAAAA482000.9476850335366466No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT418780.8233849343246407No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT412840.8117059942847906No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA400490.7874240229898166No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG327350.6436197006809569Illumina Paired End PCR Primer 2 (95% over 22bp)
TCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA316570.6224245872753033Illumina PCR Primer Index 2 (95% over 22bp)
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT305600.6008559050804962Illumina Paired End PCR Primer 2 (95% over 21bp)
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT287380.5650326243522023Illumina Paired End PCR Primer 2 (95% over 21bp)
GTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG285790.5619064434324444Illumina Paired End PCR Primer 2 (95% over 22bp)
ACAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA278010.5466097845923715Illumina Paired End PCR Primer 2 (95% over 22bp)
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT273100.5369559806200377Illumina PCR Primer Index 2 (95% over 21bp)
CTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT264710.5204599693516301Illumina Paired End PCR Primer 2 (95% over 21bp)
CCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT264400.5198504623798534Illumina PCR Primer Index 2 (95% over 21bp)
CCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG263950.5189656941950163Illumina PCR Primer Index 2 (95% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAAAAAAAA256540.5043965114180318No Hit
CTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG254260.49991368594819036Illumina PCR Primer Index 1 (95% over 22bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA241850.47551374556190446No Hit
TTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT236110.4642280358264266Illumina Paired End PCR Primer 2 (95% over 21bp)
GGAACTACAAGACACGTGCTGAAGCCAAAAAAAAAAAAAAA234000.4600794561153014No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA232680.45748413610644584No Hit
ATAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG230810.45380743276056723Illumina Paired End PCR Primer 2 (95% over 22bp)
GCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA227060.4464343645535912Illumina PCR Primer Index 2 (95% over 22bp)
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT224810.4420105236294056No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA222820.43809788210090367No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAA212320.4174532911213709No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT207030.4070523495707301No Hit
TTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG205400.40384752259009793Illumina Paired End PCR Primer 2 (95% over 22bp)
CCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT190850.3752400179470311No Hit
GTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA189280.3721531600577105Illumina PCR Primer Index 1 (95% over 22bp)
CTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT181940.35772160788725615No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT181160.35618800970020514No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT176830.3476745736105502No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA174740.34356531692986225No Hit
CTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA168920.3321223150726355Illumina PCR Primer Index 2 (95% over 22bp)
CCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA164950.3243166935308503Illumina PCR Primer Index 1 (95% over 22bp)
GGAACTACAAGACACGTGCTGAAGCAAAAAAAAAAAAAAAA162500.3194996223022926No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGAAAAAAAAAAA146590.2882181515894959No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT145700.2864682767350402No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT138440.27219401668633475No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAAAAAAAAA134980.26539113242069823No Hit
ATAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA124890.24555266356512817Illumina Paired End PCR Primer 2 (95% over 22bp)
ACAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC123790.2433898968910819No Hit
TCAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA120220.23637073595804076Illumina Paired End PCR Primer 2 (95% over 22bp)
TTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA117340.2307082195750832Illumina PCR Primer Index 1 (95% over 22bp)
TCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC115720.22752305410966958No Hit
GCTGTTGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG115370.22683490107701848Illumina Single End Adapter 2 (100% over 34bp)
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA110250.21676820528509394No Hit
AACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAA109420.21513629952194993No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAA104390.2052465573669928No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTT102570.20166816159720713No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTT102110.2007637318971514No Hit
ACAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA101930.20040982462321655Illumina Paired End PCR Primer 2 (95% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA100630.19785382764479822No Hit
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTT97700.19209300368574764No Hit
ACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAAA97180.1910706048943803No Hit
GCAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA93160.18316667577650206Illumina PCR Primer Index 2 (95% over 22bp)
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTT92130.18114153970898597No Hit
ACAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGT85010.1671425408733409No Hit
CTAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA84790.16670998753853167Illumina Paired End PCR Primer 2 (95% over 22bp)
TCAACTTCTTAGAGGGACAAGTGGCAAAAAAAAAAAAAAAA83320.16381974480139705No Hit
GTAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA82760.16271869994915533Illumina PCR Primer Index 1 (95% over 22bp)
ACAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTTG81810.16085085600338805Illumina Single End Adapter 1 (95% over 21bp)
TCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTTG81250.1597498111511463Illumina Single End Adapter 1 (95% over 21bp)
CTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTT79770.15683990689879312No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA78950.1552276626508677No Hit
ACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAA78580.15450018658777942No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA75270.14799222505042198No Hit
TCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGT72540.14262463139574344No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTCCAAAAAAAAAAAA68990.13564479349313951No Hit
TGGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAA67930.13356067287996762No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT67700.13310845802993976No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT66950.13163384438854459No Hit
ACAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG65510.1288025861970658No Hit
ACAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGC64700.12721000346435898No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA63520.12488994466856387No Hit
CCAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA63490.12483096012290806Illumina Paired End PCR Primer 2 (95% over 22bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA62700.12327770042063846No Hit
ACAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTTG62410.12270751647929898Illumina Single End Adapter 1 (95% over 21bp)
CTAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAA61930.12176376374880606Illumina Paired End PCR Primer 2 (95% over 22bp)
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGAAAAAAAAA61290.12050542677481549No Hit
TTAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA60770.11948302798344816Illumina Paired End PCR Primer 2 (95% over 22bp)
CCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT59910.11779213767464831No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGTTT59850.11767416858333671No Hit
ACAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA59630.11724161524852746No Hit
CCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTT59340.11667143130718798No Hit
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTT59340.11667143130718798No Hit
ACAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGT59090.1161798934267229No Hit
TTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTT58370.11476426433098352No Hit
TCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGC58100.11423340342008126No Hit
GCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC57490.11303405099174649No Hit
TCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTTG57460.11297506644609068Illumina Single End Adapter 1 (95% over 21bp)
ATAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA57320.11269980523303023Illumina Paired End PCR Primer 2 (95% over 22bp)
CTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTTG56750.1115790988655699Illumina Single End Adapter 1 (95% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA55940.10998651613286307No Hit
CTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT55310.10874784067409111No Hit
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGTTT55280.1086888561284353No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAA55270.10866919461321671No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTT55260.1086495330979981No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT54230.10662439703048203No Hit
CGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG53730.10564132126955189Illumina PCR Primer Index 1 (95% over 22bp)
TCAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG52840.10389144641509626No Hit
TGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAA52630.10347855459550562No Hit
GGAACTACAAGACACGTGCTGAAGTAAAAAAAAAAAAAAAA52570.103360585504194No Hit
ATAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC52500.10322295489766378No Hit
ACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAA52390.10300667823025915No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAA52000.10223987913673366No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTTG51760.10176800277148718Illumina Single End Adapter 1 (95% over 21bp)
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGTT51650.10155172610408256No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA51640.10153206458886396No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTT51610.10147308004320814No Hit
CCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC51470.1011978188301477No Hit
TCAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAAA51280.10082425004099425Illumina Paired End PCR Primer 2 (95% over 22bp)
TCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGT51200.10066695791924543No Hit
ACAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTG51010.10029338913009199No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT51010.10029338913009199No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)