FastQCFastQC Report
Mon 26 Jun 2023
SRR074361.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074361.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6548774
Total Bases268.4 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAA3878375.922284079432273No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA1698552.593691582577136No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA1631702.4916114069595316No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA1570252.3977770495668347No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA1464452.236220092493648No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA1132891.7299268534843315No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA1020331.558047353596261No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA1006571.5370357871564968No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA902421.377998385652032No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAA678761.0364688108033657No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA651750.9952244496450786No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA642800.9815577694389821No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA634010.9681354097728826No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA621860.9495823187668408No Hit
GGAACTACAAGACACGTGCTGAAGCCAAAAAAAAAAAAAAA568780.8685289796227508No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAA413890.6320114268716557No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA398240.6081138240531739No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAAAAAAAA390090.5956687465470636No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAAAAAAAAAAAAAAA372620.5689919975861131No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA371230.5668694628948869No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA366880.5602269982137115No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA355400.5426969994689083No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA345740.5279461468665738No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAA282870.43194344468140144No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAA238010.3634420732796704No Hit
AGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAAAAAAA220640.3369180246562181No Hit
AAGCCATGCATGTCTAAGTACGCACGGAAAAAAAAAAAAAA215570.329176117545055No Hit
ACGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA209150.319372755877665No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA204630.3124707006227425No Hit
TCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAAA191600.2925738466467159No Hit
GACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAA177940.27171498054445No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAAAA169440.2587354518570957No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA168810.2577734397308565No Hit
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCAAAAAAAAAAAA162980.2488710100547064No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA162440.24804642823221568No Hit
TCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAAAAAA158800.24248813594727808No Hit
AATCAGTTATGGTTCCTTTGGTCGCCCAAAAAAAAAAAAAA157720.24083897230229656No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAA152330.23260842411113894No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAAAAAAAAA150340.2295696873949231No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAA148180.2262713601049601No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAA145590.22231642136375449No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAA135930.20756556876142007No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAA130790.1997167714140082No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAA129680.1980217976677772No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA128830.19672384479904179No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA125490.19162365352659902No Hit
AACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAA124640.19032570065786358No Hit
GAGGGGCCCGTGCCTTGGAAAGCGTCGCAAAAAAAAAAAAA124500.190111920185366No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAA124130.18954692893662234No Hit
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT118860.18149962115046267Illumina PCR Primer Index 2 (95% over 21bp)
CTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAAAA112870.17235287093431534No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAA112500.1717878796855717No Hit
TCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG111760.17065789718808436Illumina Paired End PCR Primer 2 (95% over 22bp)
AGGGGCCCGTGCCTTGGAAAGCGTCGCAAAAAAAAAAAAAA106870.1631908506844182No Hit
GAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAA106770.1630381503469199No Hit
GGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAA106130.16206086818693086No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG104960.16027427423820093Illumina Paired End PCR Primer 2 (95% over 22bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAA103000.1572813476232345No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT102990.1572660775894847No Hit
GGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAA101400.15483814222326195No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA100380.15328059878077943No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT99790.15237966678953954Illumina PCR Primer Index 1 (95% over 21bp)
TACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAAA99370.15173832537204673No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA99320.15166197520329758No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCGAAAAAAAAAAA98590.1505472627395601No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAA97720.14921876980332502No Hit
AGAGTTCTACAGTCCGACGATCACGCAAAAAAAAAAAAAAA97320.14860796845333188Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACGAGACTCTGGCATGCTAACTAGTTACAAAAAAAAAAAAA96980.14808878730583772No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAA96010.14660759403210433No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT95960.1465312438633552Illumina Paired End PCR Primer 2 (95% over 21bp)
GCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA94990.1450500505896218Illumina PCR Primer Index 2 (95% over 22bp)
ACAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG91440.13962918860843265Illumina Paired End PCR Primer 2 (95% over 22bp)
CGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAAA89790.13710963303971094No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCAAAAAAAAAAAA88910.13576587006972604No Hit
GTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAAAA85430.13045189832478568No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT84940.12970366667104408No Hit
GTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG84810.1295051562322963Illumina Paired End PCR Primer 2 (95% over 22bp)
GCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAAAA83360.12729100133857116No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAAAAAAAAAAAAAAA82930.12663438988732853No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA82430.1258708881998371No Hit
TGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAA82410.12584034813233744No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAA80380.12274053128112225No Hit
AGGGGCCCGTGCCTTGGAAAGCGTCGCGAAAAAAAAAAAAA79830.12190067942488166No Hit
TCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA78610.12003773530740258Illumina PCR Primer Index 2 (95% over 22bp)
ACCCGTCGCCGCGCTCTCTCTCTCCCCCAAAAAAAAAAAAA78580.1199919252061531No Hit
GTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA76510.11683102821993857Illumina PCR Primer Index 1 (95% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAA74900.11437255278621616No Hit
AAATCAGTTATGGTTCCTTTGGTCGCCCAAAAAAAAAAAAA74380.1135785110312251No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT73360.11202096758874257Illumina PCR Primer Index 2 (95% over 21bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAAAAAAAA73070.11157813660999753No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAA71990.10992897296501604No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT69840.10664591570880291No Hit
AGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA68970.10531742277256782No Hit
GAGGGGCCCGTGCCTTGGAAAGCGTCGCGAAAAAAAAAAAA66400.10139302409886188No Hit
TGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAA65860.10056844227637111No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAA65800.10047682207387214No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)