FastQCFastQC Report
Mon 26 Jun 2023
SRR074362.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074362.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343046
Total Bases55 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAA14816011.03164001828679No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA549434.09092465931919No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA392542.9227591608924786No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA331122.4654404986873124No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA309242.3025272403179042No Hit
ACGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA298662.223751085219717No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA265521.9769985540331456No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAA202351.506649809462967No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA195001.4519234635299163No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA157041.169282362629426No Hit
ACGAGACTCTGGCATGCTAACTAGTTACAAAAAAAAAAAAA128920.9599075534270606No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA127790.9514938430999385No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA124570.9275184915483162No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA122980.9156797309995339No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA121820.9070426478318688No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA94600.7043690238457954No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAA93080.6930514665916134No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA79930.5951397048202369No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA77180.5746638611037895No Hit
TCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAAA75440.5617082363522917No Hit
ACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAA66000.4914202491947409No Hit
TAGGCACAGCTGGGTAGCTAAGTACGGAAAAAAAAAAAAAA61380.457020831751109No Hit
ATCCACCTATAACTTCTCTGTTAACCCAAAAAAAAAAAAAA60540.45076639221590326No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAA57730.4298438028183696No Hit
ACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAA55660.4144310768208982No Hit
TACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAA55050.4098891623965225No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAA49630.3695331358717423No Hit
ACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAA49540.3688630173501131No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAA45900.341760446030888No Hit
ATCGCATGATGAGTAATAGAAAGGAGCAAAAAAAAAAAAAA45280.337144073992998No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA40250.2996918943952776No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA36860.2744507634139114No Hit
AATCCACCTATAACTTCTCTGTTAACCCAAAAAAAAAAAAA35900.2673028325165333No Hit
ACGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAA35260.2625375452516146No Hit
TCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG35110.26142068104889926Illumina Paired End PCR Primer 2 (95% over 22bp)
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT34760.25881466457589686Illumina PCR Primer Index 2 (95% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA34390.25605973287586575No Hit
ATGTACACGGAGTCGACCCACAACGCAAAAAAAAAAAAAAA31040.23111643234855692No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAAAA30780.22918053439718372No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAAAAAAAAAAAA30640.22813812780798276No Hit
TTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT30480.22694680599175307Illumina Paired End PCR Primer 2 (95% over 21bp)
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT29960.22307501008900663No Hit
GCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA29340.21845863805111665No Hit
ACGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAAA28350.2110873343131955No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAA27870.2075133688645065No Hit
TTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG27410.20408831864284618Illumina Paired End PCR Primer 2 (95% over 22bp)
ACGAGACTCTGGCATGCTAACTAGTTACGAAAAAAAAAAAA27040.20133338694281505No Hit
TCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA27030.2012589293293007Illumina PCR Primer Index 2 (95% over 22bp)
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT26600.19805725194818347Illumina PCR Primer Index 1 (95% over 21bp)
ACAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG26270.19560015070220976Illumina Paired End PCR Primer 2 (95% over 22bp)
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA25830.19232401570757815No Hit
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT25810.19217510048054942Illumina Paired End PCR Primer 2 (95% over 21bp)
TCCACCTATAACTTCTCTGTTAACCCAAAAAAAAAAAAAAA24740.18420813583451348No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA23910.17802815391282203No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA23670.17624117118847754No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA23140.17229491767221675No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA22860.1702101044938148No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT22460.16723179995324064No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAA21880.16291325836940804No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA21690.16149856371263532No Hit
ATAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG21570.16060507235046304Illumina Paired End PCR Primer 2 (95% over 22bp)
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT21350.15896700485314724No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG20390.1518190739557692Illumina Paired End PCR Primer 2 (95% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA20200.15040437929899644No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT20020.14906414225573808No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA20020.14906414225573808No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAAAAA19790.1473516171449079No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAA19760.14712824430436486No Hit
TTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA19420.1445966854448768Illumina PCR Primer Index 1 (95% over 22bp)
ACAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA19380.14429885499081938Illumina Paired End PCR Primer 2 (95% over 22bp)
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA18880.14057597431510163No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCCAAAAAAAAAAA18710.13931019488535762No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAAAAAAAA18430.1372253817069557No Hit
TCAACTTCTTAGAGGGACAAGTGGCAAAAAAAAAAAAAAAA18410.13707646647992697No Hit
CCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAAA18370.13677863602586954No Hit
GCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA18110.13484273807449632Illumina PCR Primer Index 2 (95% over 22bp)
AAGGCACAGCTGGGTAGCTAAGTACGGAAAAAAAAAAAAAA17990.13394924671232408No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAAAAA17630.1312687726258073No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT17050.12695023104197473Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAAA16630.12382301127437183No Hit
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAA16490.12278060468517088No Hit
ATGTACACGGAGTCGACTCGCAACGCAAAAAAAAAAAAAAA16450.12248277423111346No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAA16220.1207702491202833No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA16180.12047241866622586No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA16130.1201001305986541No Hit
AGAGTTCTACAGTCCGACGATCACGCAAAAAAAAAAAAAAA15690.1168239956040225Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG15520.11555821617427846Illumina PCR Primer Index 2 (95% over 22bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAA14870.11071847129584542No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAA14730.10967606470664445No Hit
AAATCCACCTATAACTTCTCTGTTAACCCAAAAAAAAAAAA14570.10848474289041478No Hit
ATAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA14460.10766570914175688Illumina Paired End PCR Primer 2 (95% over 22bp)
CCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT14380.10707004823364204Illumina PCR Primer Index 2 (95% over 21bp)
ACCTATAACTTCTCTGTTAACCCAAAAAAAAAAAAAAAAAA14250.10610209925795544No Hit
ACGAGCGCAACCCTTTTCTTTAGTTACTAAAAAAAAAAAAA14070.10476186221469704No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAAA13720.10215584574169462No Hit
TCGACGTACGCAGTTTTATCCGGTAAAACAAAAAAAAAAAA13470.10029440540383575No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)