FastQCFastQC Report
Mon 26 Jun 2023
SRR074363.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074363.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3252925
Total Bases133.3 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAA1672665.142018337342546No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAA1308384.02216466718415No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA982603.0206660159702423No Hit
TGGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAA972942.9909696657623526No Hit
GGAACTACAAGACACGTGCTGAAGCCAAAAAAAAAAAAAAA499401.535233674308507No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA397161.2209319304933253No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA386261.187423626428522No Hit
TGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAA371451.1418953710890967No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAA360471.1081411345173957No Hit
ACGAGACTCTGGCATGCTAACTAGTTACAAAAAAAAAAAAA292910.9004511324423403No Hit
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGAAAAAAAAA285460.8775486677374978No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA263990.8115465311988441No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAA227060.6980179377022219No Hit
TGGAACTACAAGACACGTGCTGAAGCCAAAAAAAAAAAAAA214560.6595909834994659No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGAAAAAAAAAAA206370.6344136431058202No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAA205750.6325076661773634No Hit
AGGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAA204080.6273738250958752No Hit
AACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAA201260.6187047042277335No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA187720.5770806274353083No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA183620.5644765864568042No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAA176090.5413281892450641No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA167900.5161508488514184No Hit
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAA160870.49453952980778837No Hit
GCTGTTGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG141350.4345319981247647Illumina Single End Adapter 2 (100% over 34bp)
TCTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAAA140440.43173451585880396No Hit
GGAACTACAAGACACGTGCTGAAGCAAAAAAAAAAAAAAAA139770.42967483111353627No Hit
ACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAAA137480.4226350131035914No Hit
GGAACTACAAGACACGTGCTGAAGGCAAAAAAAAAAAAAAA134990.41498036382640235No Hit
GGGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAA132280.40664940015524487No Hit
GGAACTACAAGACACGTGCTGAAGTAAAAAAAAAAAAAAAA110080.33840312949115026No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA103450.3180214729820085No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAA101100.3107972055918904No Hit
AGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAA98150.30172844440004No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT88590.2723395098257722Illumina PCR Primer Index 1 (95% over 21bp)
TACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAAAAA88540.27218580200896114No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA87320.26843533127877217No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA87090.2677282753214415No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA85760.26363964739426826No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCAAAAAAAAAAAA84800.2606884573114966No Hit
TGAACTACAAGACACGTGCTGAAGCCAAAAAAAAAAAAAAA84100.2585365478761423No Hit
GAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAA83370.2562924137507013No Hit
ACGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA82740.2543556952588824No Hit
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT81210.24965223606446507Illumina PCR Primer Index 2 (95% over 21bp)
ACAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG80560.24765403444592174Illumina Paired End PCR Primer 2 (95% over 22bp)
GGAACTACAAGACACGTGCTGAAGACAAAAAAAAAAAAAAA76130.234035521876465No Hit
TCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG75910.2333592074824965Illumina Paired End PCR Primer 2 (95% over 22bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA75320.23154545524412645No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA75290.23145323055403982No Hit
TGGAACTACAAGACACGTGCTGAAGGCAAAAAAAAAAAAAA75220.23123803961050438No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA72150.2218003796583075No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT71670.2203247846169217No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT68100.20935004649661457No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAA67670.20802815927203977No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA65480.20129575689571694No Hit
AACGAGACTCTGGCATGCTAACTAGTTACAAAAAAAAAAAA62430.19191958007024448No Hit
ACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAA60970.18743131181936257No Hit
ACTACAAGACACGTGCTGAAGCCAAAAAAAAAAAAAAAAAA59500.18291230200511846No Hit
TCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA57310.17617989962879563Illumina PCR Primer Index 2 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA56230.1728598107856775No Hit
AACTACAAGACACGTGCTGAAGCCAAAAAAAAAAAAAAAAA55940.17196830544817357No Hit
TGGAACTACAAGACACGTGCTGAAGACAAAAAAAAAAAAAA53110.16326844301666962No Hit
ACAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA52850.1624691623692523Illumina Paired End PCR Primer 2 (95% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAAAAAAAA52760.16219248829899247No Hit
ACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAA51200.1573968044144885No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG48590.14937325637695303Illumina Paired End PCR Primer 2 (95% over 22bp)
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT48070.1477746950821184Illumina Paired End PCR Primer 2 (95% over 21bp)
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA47890.14722134694159872No Hit
TTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT45380.1395052145376853Illumina Paired End PCR Primer 2 (95% over 21bp)
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT44740.13753775448250422Illumina Paired End PCR Primer 2 (95% over 21bp)
CCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG44530.1368921816518979Illumina PCR Primer Index 2 (95% over 22bp)
CCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT42720.13132795868333885Illumina PCR Primer Index 2 (95% over 21bp)
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA42260.1299138467686774No Hit
GCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA42180.1296679142617798Illumina PCR Primer Index 2 (95% over 22bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAA41950.1289608583044491No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT41280.12690117355918135No Hit
TGGAACTACAAGACACGTGCTGAAGCAAAAAAAAAAAAAAA41200.1266552410522837No Hit
TGGAACTACAAGACACGTGCTGAAGTAAAAAAAAAAAAAAA41160.1265322747988349No Hit
CTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT40030.12305847813890575Illumina Paired End PCR Primer 2 (95% over 21bp)
ATAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG40010.12299699501218135Illumina Paired End PCR Primer 2 (95% over 22bp)
AGGAACTACAAGACACGTGCTGAAGCCAAAAAAAAAAAAAA39780.12228993905485062No Hit
TTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG39290.12078360245010261Illumina Paired End PCR Primer 2 (95% over 22bp)
CTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG38130.11721758110008684Illumina PCR Primer Index 1 (95% over 22bp)
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT35550.109286257752638No Hit
ACAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC35410.10885587586556714No Hit
CCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT34620.10642729235995298No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGAAAAAAAAAAAA34560.10624284297977975No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA34460.1059354273461577No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA34350.10559727014917343No Hit
CTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA33820.10396796729097658Illumina PCR Primer Index 2 (95% over 22bp)
CCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA33320.10243088912286634Illumina PCR Primer Index 1 (95% over 22bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAAAAAAAAA33140.10187754098234666No Hit
TACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAAAAT32630.1003097212508742No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT32570.100125271870701No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)