FastQCFastQC Report
Mon 26 Jun 2023
SRR074364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6408570
Total Bases262.7 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA1884412.9404531744211266No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA1675472.614421001877174No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAA921571.4380275162789826No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA884971.3809164915105867No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA677011.0564135212691754No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA671831.0483305948128834No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA580550.9058963232047086No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA579500.9042578921662711No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG498960.7785824294655438Illumina Paired End PCR Primer 2 (95% over 22bp)
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA471540.7357959732046307No Hit
GTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG468570.7311615539816215Illumina Paired End PCR Primer 2 (95% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA460420.7184442083023203No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA457280.7135445192921354No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT440830.6878757663566131Illumina Paired End PCR Primer 2 (95% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA436300.6808071067336395No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA413260.6448552485187803No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT406610.6344785186086755Illumina PCR Primer Index 2 (95% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAA403940.6303122225395057No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA381520.5953278188425811No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT365620.5705172916890976Illumina PCR Primer Index 1 (95% over 21bp)
GCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA359630.5611704327174393Illumina PCR Primer Index 2 (95% over 22bp)
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT359270.5606086849328321Illumina PCR Primer Index 2 (95% over 21bp)
TCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG344250.5371713190306107Illumina Paired End PCR Primer 2 (95% over 22bp)
GTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA337650.5268726096461457Illumina PCR Primer Index 1 (95% over 22bp)
ACAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG335210.5230652079949193Illumina Paired End PCR Primer 2 (95% over 22bp)
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT287720.44896131274215617No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA286670.44732288170371864No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA283270.4420174859602064No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT272760.42561757147070256No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA262900.4102319238145171No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT261440.4079537244658325No Hit
GGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAA259480.404895319860749No Hit
CTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG254340.3968748098249687Illumina PCR Primer Index 1 (95% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAA253270.3952051705762752No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA238660.3724075729843007No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT237030.36986410384844043No Hit
CTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT234390.36574462009465447Illumina Paired End PCR Primer 2 (95% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAA226370.3532301277820169No Hit
TCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA210160.3279358733695661Illumina PCR Primer Index 2 (95% over 22bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAA209750.327296105059319No Hit
ACAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA197100.307556912072428Illumina Paired End PCR Primer 2 (95% over 22bp)
CTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA174380.27210438522166414Illumina PCR Primer Index 2 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAA173560.27082484860117No Hit
CCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG165040.25753015103213356Illumina PCR Primer Index 2 (95% over 22bp)
TGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA165020.2574989428218776No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA158790.2477775853271479No Hit
CCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT154380.24089617496570997Illumina PCR Primer Index 2 (95% over 21bp)
GCAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA149590.23342180860940898Illumina PCR Primer Index 2 (95% over 22bp)
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT148140.2311592133658523Illumina Paired End PCR Primer 2 (95% over 21bp)
CTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT146080.22794476770948902No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA144580.22560415194029246No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA139080.21702189411990508No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAAAAAAAAAAAAAAA138550.21619487654812225No Hit
ATAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG135130.21085827259435413Illumina Paired End PCR Primer 2 (95% over 22bp)
GTAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA127680.19923321427401122Illumina PCR Primer Index 1 (95% over 22bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAAAAAAAAAAAAAA123390.19253905317410905No Hit
CGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG118780.1853455607101116Illumina PCR Primer Index 1 (95% over 22bp)
AAGCCATGCATGTCTAAGTACGCACGGAAAAAAAAAAAAAA118530.1849554580819122No Hit
TTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT117770.18376954609218593Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAAA115640.18044587169992682No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAA115270.17986851981019167No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAA114620.17885425297687316No Hit
CGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA110400.17226932061286684Illumina Paired End PCR Primer 2 (95% over 22bp)
CCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA108810.1697882678975185Illumina PCR Primer Index 1 (95% over 22bp)
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT106430.16607449087705994No Hit
TTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG105260.16424881057708662Illumina Paired End PCR Primer 2 (95% over 22bp)
CCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT104780.16349981353094373No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAA102900.16056624176688403No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT94680.14773966735168687No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT94480.14742758524912736No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAA87400.13637987881851957No Hit
TCAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA84500.1318546883314062Illumina Paired End PCR Primer 2 (95% over 22bp)
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT84450.13177666780576633No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT83730.13065317223655198No Hit
GGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG83520.13032548602886448Illumina Paired End PCR Primer 2 (95% over 22bp)
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCAAAAAAAAAAAA82440.12864024267504295No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAA80270.12525415186227193No Hit
GACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAA80030.12487965333920048No Hit
ACAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC79820.12455196713151297No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA79350.12381857419049803No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTT79260.12367813724434626No Hit
CGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT78250.12210212262642056Illumina Paired End PCR Primer 2 (95% over 21bp)
CTAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA77850.12147795842130148Illumina Paired End PCR Primer 2 (95% over 22bp)
ATAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA77130.12035446285208712Illumina Paired End PCR Primer 2 (95% over 22bp)
GCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC76890.11997996432901567No Hit
TGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG76520.11940261243928052Illumina Paired End PCR Primer 2 (95% over 22bp)
ACAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGAA76460.11930898780851265Illumina Paired End PCR Primer 2 (95% over 22bp)
GGAACTACAAGACACGTGCTGAAGCCAAAAAAAAAAAAAAA76030.11863801128800965No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTTG75570.11792022245212269Illumina Single End Adapter 1 (95% over 21bp)
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTT72570.11323899091372958No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCT72500.11312976217783374No Hit
TCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC71270.11121045724709258No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAA71270.11121045724709258No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAAA71090.11092958335478897No Hit
TCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAAA70770.11043025199069371No Hit
ACGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA70750.11039904378043776No Hit
GGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA70190.10952521389327105Illumina Paired End PCR Primer 2 (95% over 22bp)
GTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTTG70060.10932236052660733Illumina Single End Adapter 1 (95% over 21bp)
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAA69610.10862017579584837No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAA69510.1084641347445686No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTT68670.10715338991381854No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA68310.10659164212921135No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAA67180.10482837824974994No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGTTT66870.10434465099078266No Hit
AGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA66190.10328357184208022No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTTTGCTT65910.10284665689849685No Hit
GGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT65730.10256578300619328Illumina PCR Primer Index 2 (95% over 21bp)
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA64280.10030318776263661No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)