FastQCFastQC Report
Mon 26 Jun 2023
SRR074365.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074365.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2585299
Total Bases105.9 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA1837187.106257341994097No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA1410975.457666598718369No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA930063.597494912580711No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA769912.978030780965761No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA590012.2821731644966405No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAA545152.1086535832025617No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA509311.9700235833456787No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA488071.8878667419126374No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA421781.631455394521098No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA387741.4997878388534556No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA288901.1174722923731453No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA266881.032298391791433No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA266061.029126611660779No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA236080.913163235664424No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA195820.7574365672984054No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA191680.7414229456631515No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAA179680.695006651068213No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA165440.6399259814822192No Hit
ACGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA164510.6363287186511115No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA142530.5513095390513825No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAA133910.5179671674340183No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA133690.517116202033111No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA131870.5100763973528788No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAAA123380.4772368689269597No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA119390.4618034509741426No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA118230.4573165424966319No Hit
ACGAGACTCTGGCATGCTAACTAGTTACAAAAAAAAAAAAA111660.4319036212059031No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA102090.3948866262664396No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAA98390.3805749354330002No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTAAAAAAAAAAAAAA84420.3265386324753926No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAA83270.32209040424337765No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAA82350.31853182165776567No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAAAA80940.3130779070428604No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA77960.30155119388511736No Hit
CTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAA75980.2938925052769525No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA75580.2923452954571212No Hit
ACGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAA69300.26805410128577006No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAA67070.2594284065402106No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAAAA66500.2572236325469511No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAA66430.25695287082848056No Hit
CCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAAA66050.25548302149964086No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAA63730.2465092045446194No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGAAAAAAAAAAAA62530.24186757508512557No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAAAAA62350.24117133066620144No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAAAA57840.22372653994760372No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA56710.21935567220658037No Hit
ACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAA56100.21699617723133766No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA55130.2132441934182468No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA54940.21250926875382692No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTAAAAAAAAAAAAAA54190.20960825034164327No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA53960.20871860469524028No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAA53470.20682327266594697No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA53120.20546946407359457No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAA52810.20427037646322532No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA52140.20167880001500793No Hit
ACGCGCGCTACACTGACTGGCTCAGCGAAAAAAAAAAAAAA51530.1993193050397652No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA51470.1990872235667905No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA49550.19166061643160037No Hit
CGTGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA48200.18643878328966978No Hit
CTAAACCATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA48090.1860133005892162Illumina PCR Primer Index 1 (95% over 22bp)
ACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAAAAAAAAA46260.17893481566348804No Hit
CTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAAAAAAA45070.17433186644948998No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA44780.1732101393301123No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAA43110.1667505383323167No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA42600.1647778458120318No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAAAAAAAA41770.1615673854358819No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAAA41110.15901448923316025No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAA40260.15572666836601878No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA39890.15429549928267486No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA38920.15054351546958397No Hit
AGGCACAGCTGGGTAGCTAAGTACGGAAAAAAAAAAAAAAA38350.1483387414763244No Hit
GCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA37790.1461726477285606No Hit
TAGGCACAGCTGGGTAGCTAAGTACGGAAAAAAAAAAAAAA37750.1460179267465775No Hit
AACGAGACTCTGGCATGCTAACTAGTTACAAAAAAAAAAAA36740.14211122195150347No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAA36540.14133761704158784No Hit
TGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAAA36540.14133761704158784No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAA35480.13723751101903495No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAA35460.13716015052804337No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA35220.13623182463614458No Hit
TCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAAAA35090.13572898144469941No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAA34760.13445253334333862No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAA33700.13035242732078572No Hit
AAGCTTGCGTTGATTAAGTCCCTGCCCTAAAAAAAAAAAAA33610.1300043051113237No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAA33500.12957882241087007No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGAAAAAAAAAAA33130.12814765332752615No Hit
TCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAAA33080.1279542521000472No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA32620.12617496080724125No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAA31720.12269373871262088No Hit
TCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAAA31690.12257769797613352No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAAA31640.12238429674865461No Hit
CGTGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAAA31440.12161069183873896No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA31270.12095312766531068No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAA30710.11878703391754687No Hit
TCAACTTCTTAGAGGGACAAGTGGCAAAAAAAAAAAAAAAA30660.11859363269006795No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAA30620.11843891170808483No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA30490.11793606851663967No Hit
TTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA29870.11553789329590118No Hit
AAGCCATGCATGTCTAAGTACGCACGGAAAAAAAAAAAAAA29830.11538317231391804No Hit
CCGCCGAGGGCGCACCACTGGCCCGTCTCGCAAAAAAAAAA29640.11464824764949819No Hit
CGTGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAAAA29250.11313971807516267No Hit
ACGTGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAA28920.11186326997380186No Hit
ACCCCGAGTGTTACAGCCCTCCGGAAAAAAAAAAAAAAAAA28330.10958113548955072No Hit
GGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAA28270.10934905401657603No Hit
TATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAAA27620.1068348380593502No Hit
CCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA27520.10644803560439238No Hit
TTATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAA27460.10621595413141768No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA27320.10567443069447673No Hit
TCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAAAAAA26610.1029281332642762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)