FastQCFastQC Report
Mon 26 Jun 2023
SRR074366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531516
Total Bases62.7 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGACTCTGGCATGCTAACTAGTTACAAAAAAAAAAAAA1071256.994703287461575No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA564443.6854985517617838No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA482643.151387252891906No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA459723.0017316175606394No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA459332.9991851211479346No Hit
TCAACTTCTTAGAGGGACAAGTGGCAAAAAAAAAAAAAAAA240911.573016540473622No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA239191.561785838345796No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAA237071.5479433450254518No Hit
AACGAGACTCTGGCATGCTAACTAGTTACAAAAAAAAAAAA220391.439031652297462No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA211611.3817028356216976No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA190391.2431473128586314No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGAAAAAAAAAAAA164101.071487336730403No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA150290.981315245808728No Hit
GGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAA143930.939787765847696No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA123890.8089370271025572No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA119550.7805990926637397No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA118840.7759631632970208No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA114500.7476252288582033No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA97340.6355793866991922No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTAAAAAAAAAAAAAA88870.5802747081976289No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA79280.5176570143570162No Hit
ACAACTTCTTAGAGGGACAAGTGGCAAAAAAAAAAAAAAAA75620.4937591249454788No Hit
TCAACTTCTTAGAGGGACAAGTGGCGAAAAAAAAAAAAAAA73910.4825937175974655No Hit
TGGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAA73040.4769130717537394No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAA72910.47606423961617117No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA70810.462352335855453No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA57330.3743349726676052No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAA50530.3299345223948036No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAAAA49990.3264086042849046No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA49690.3244497608905163No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA47760.31184786838661827No Hit
CTAAACCATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA47470.30995431977204285Illumina PCR Primer Index 1 (95% over 22bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA47280.3087137189555969No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAA44170.2884070424337715No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA44030.2874929155163903No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAA42280.27606632904912515No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA39770.25967733931607634No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA38680.25256020831646553No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA38670.25249491353665254No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAA36090.2356488603449131No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGAAAAAAAAAAA35230.23003350928099997No Hit
GGAACTACAAGACACGTGCTGAAGCCAAAAAAAAAAAAAAA34820.22735642330866931No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAAA33980.22187166180438206No Hit
ACGAGACTCTGGCATGCTAACTAGTTAAAAAAAAAAAAAAA33620.2195210497311161No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCAAAAAAAAAAAAAA33030.2156686577221524No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAA31780.20750681024553447No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAA30550.19947555232854244No Hit
AGAATTCACCAAGCGTTGGATTGTTCAAAAAAAAAAAAAAA30000.19588433943883055No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAAAA29600.1932725482463128No Hit
TGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAA28400.18543717466875959No Hit
TAACGAGACTCTGGCATGCTAACTAGTTACAAAAAAAAAAA27950.18249890957717713No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA27430.17910358102690405No Hit
ACAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA27350.17858122278840052No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAAA25880.16898289015589782No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAAAAA25600.1671546363211354No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAA24830.16212693827553876No Hit
TGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAAA24760.16166987481684814No Hit
ACAACTTCTTAGAGGGACAAGTGGCGTAAAAAAAAAAAAAA23720.154879217716302No Hit
GCAACTTCTTAGAGGGACAAGTGGCAAAAAAAAAAAAAAAA23370.152593900422849No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA23130.15102682570733836No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAAAAAAAA22910.1495903405514536No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCAAAAAAAAAAAAAA21810.1424079147720298No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAA21200.13842493320344026No Hit
ACAACTTCTTAGAGGGACAAGTGGCGAAAAAAAAAAAAAAA21040.13738021672643316No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA20850.13613961590998724No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA20730.1353560785522319No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAA20590.1344419516348507No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAAAA20340.13280958213952712No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAA19970.1303936752864482No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA19860.12967543270850582No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA19690.12856542145168579No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA19540.12758599975449164No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAA19500.12732482063523987No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAA19460.1270636415159881No Hit
AACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAA19260.1257577459197292No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAA17470.11406998033321233No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAA17150.11198054737919813No Hit
GCGCCGGGCCGTACCCATATCCGCAGCAAAAAAAAAAAAAA17070.11145818914069458No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA16820.10982581964537098No Hit
AGAAACCTCCCGTGGAGCAGAAGGGCAAAAAAAAAAAAAAA16410.1071487336730403No Hit
ACGAGACTCTGGCATTCTAACTAGTTACAAAAAAAAAAAAA16400.10708343889322737No Hit
TTTGTCTTCATCATGTCTGGTCGTGGCAAAAAAAAAAAAAA16380.10695284933360148No Hit
TGGAACTACAAGACACGTGCTGAAGCCAAAAAAAAAAAAAA16170.10558165895752966No Hit
ACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAA15930.10401458424201901No Hit
AGGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAA15720.1026433938659472No Hit
GCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA15710.10257809908613426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)