FastQCFastQC Report
Mon 26 Jun 2023
SRR074367.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074367.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056997
Total Bases84.3 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA1525377.415518836439723No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA987944.802826644861416No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA959764.665830820365805No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA544252.6458473201467965No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA532182.5871695486186903No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA454522.2096288910484554No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA439492.1365612103469283No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA435772.1184765947641147No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA290151.4105514009014113No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAA277071.3469635590134552No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA245511.1935360139076527No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA224631.0920288167654109No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA184420.896549678973766No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA180140.8757426481419273No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA178890.869665828389638No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA167830.8158981272213814No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA115580.5618870615756854No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAAA112990.5492958910489417No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA111160.54039942693159No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA105100.5109390047724912No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA101930.49552818988068525No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA101170.4918334834712933No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA98530.4789992401544582No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA93990.4569282308141432No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA93400.45405997189106256No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA91030.44253832164072193No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA87720.4264469029366596No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAA85990.41803658439949115No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA83390.4053967993147292No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAA75090.3650467161595277No Hit
GGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAA71070.34550366383616504No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA70430.34239233212299286No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA64050.3113762441073079No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA62390.30330622747626756No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA60510.29416669056882433No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA60260.2929513266183665No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAA59930.29134704620376206No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA59700.29022891136934087No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAA58750.2856105283576009No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAA46130.22425895613848731No Hit
ACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAA41320.20087535373167778No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA39370.19139551491810636No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAA38570.18750635027664114No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA38460.18697159013843967No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAA37520.18240182168471805No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAA36780.17880434439136275No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA35950.1747693360758426No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAA34700.16869251632355323No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAA34360.1670396213509305No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAA33040.1606224996925129No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA29650.14414216452430412No Hit
ACGAGACTCTGGCATGCTAACTAGTTACAAAAAAAAAAAAA29140.14166282206537004No Hit
CGTGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA28720.13962101062860083No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAA28060.13641244979939202No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAA27870.13548877319704403No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA25780.1253283305712162No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTAAAAAAAAAAAAAA25490.12391850838868507No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAAAAAAAA25230.12265452988020886No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA23760.11550818985151656No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGAAAAAAAAAAA23700.11521650250340668No Hit
TTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAAAA23430.11390390943691216No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAA22870.1111814941878865No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAA22860.11113287962986819No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAA22120.10753540233651288No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAA21010.10213918639647993No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAAA20690.10058352053989383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)