FastQCFastQC Report
Mon 26 Jun 2023
SRR074368.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074368.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6490822
Total Bases266.1 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA3832715.90481452118083No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA3719135.729828980058304No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA3035874.6771733996094795No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA2982314.594656886292676No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA1060341.633598949408873No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA1002291.544164976331195No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA669201.0309942253847046No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA638880.9842821140373283No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAA635000.9783044427963054No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA615710.9485855566521466No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAA610780.9409902166474446No Hit
TGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAAA538170.8291245700467522No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAA487790.7515072821285192No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA464030.7149017489618419No Hit
CTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAA436950.6731813012281034No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA432290.6660019331912045No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAA425000.6547706900605194No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA401820.6190587263061597No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAA385830.5944239419907063No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAA367400.5660300035958465No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA327760.5049591561746725No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA322180.4963624021734074No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAA306740.4725749681627381No Hit
AAGCCATGCATGTCTAAGTACGCACGGAAAAAAAAAAAAAA305250.4702794191552318No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAAA304010.4683690293771729No Hit
TTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAAA285910.4404835011651837No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA278740.42943713446463333No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA268770.4140769843942724No Hit
CTAAACCATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA267250.41173521627923243Illumina PCR Primer Index 1 (95% over 22bp)
ACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAAAA256680.3954506840581979No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAA255640.3938484216636969No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAA248770.38326424603848325No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA248630.38304855686999273No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAA241050.37137052903314866No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA234030.36055525787026665No Hit
TATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAAAAAAA226630.34915454467862467No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA226410.348815604556711No Hit
TGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA225700.34772175234508046No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA222920.3434387817136258No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA222770.34320768617595737No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA222620.34297659063828895No Hit
AGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA221470.3412048581828311No Hit
TCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAAA221340.3410045753835184No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA217020.334349023898668No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA208450.32114576551321233No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA207620.31986703687144713No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA205370.3164006038064208No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA200070.3082352281421367No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAA187020.28812991636498425No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCAAAAAAAAAAAAAA186220.2868974068307527No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA183820.28319987822805803No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAA181580.27974885153220963No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA176200.27146022491450233No Hit
TTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAAAA172090.26512820718238767No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAA167970.25878078308109514No Hit
TGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAAAAAA167470.2580104646222004No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCAAAAAAAAAAAAAAA164810.25391237042088044No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA163140.251339506768172No Hit
ACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAA157500.2426503145518395No Hit
CTGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAAAAA156700.24141780501760793No Hit
TGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAAA156340.24086317572720373No Hit
AGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA153650.2367188624183501No Hit
ATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAAAAA150230.23144988415951018No Hit
GCTGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAAAA143490.22106599133360919No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAA142900.22015701555211345No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAA142610.2197102308459545No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA141860.21855475315761239No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA132790.20458117631326203No Hit
TGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAA123160.18974484279494955No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAA119290.18378257792310435No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA117620.18120971427039595No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAA115280.17760462388276863No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAA114420.1762796761334697No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAA112960.17403034623349709No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA111650.1720121118711929No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAA105530.1625834139343214No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA101570.15648249173987516No Hit
CTAGACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAA100620.15501888666797517No Hit
AAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG99400.15313930962827205Illumina PCR Primer Index 2 (95% over 22bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAA99070.15263089944540154No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAA96840.14919527911873104No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA94750.14597534796055106No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAAAAAAAA92940.14318679513935215No Hit
TATCCGGTAAAGCGAATGATTAGAGGTCAAAAAAAAAAAAA92470.14246269578799112No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA92100.141892660128409No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAA91640.14118396714622586No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA91120.14038283594897535No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA90960.14013633404212902No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT90530.13947386016747956No Hit
GCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAA88910.13697802836066064No Hit
GAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAAAA87650.13503682584424592No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAAAAAAAA87640.13502141947506802No Hit
TTACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAA87110.1342048819086396No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAA86990.13402000547850487No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAA86370.13306481058947542No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAA85620.13190933290113332No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA85470.1316782373634649No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA84040.12947512657102597No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAA83400.12848911894364073No Hit
GGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAA83270.1282888361443281No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA82460.12704092024091862No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA78940.12161787829029974No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA77570.1195072057129282No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAA76350.11762762867322506No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAA74650.11500854591298297No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA74440.11468501216024718No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA74110.11417660197737667No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA74050.1140841637623093No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAA73810.11371441090203982No Hit
AAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA70780.10904628104113777Illumina Paired End PCR Primer 2 (95% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCGAAAAAAAAAA69710.10739779953910306No Hit
CTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAA68970.10625772821993885No Hit
TTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAAAAA68920.1061806963740494No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA68540.1055952543452894No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAA67780.10442437028776941No Hit
GCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA66940.10313023527682627No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAAA66850.10299157795422521No Hit
AGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAA66390.10228288497204208No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA66300.102144227649441No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)