FastQCFastQC Report
Mon 26 Jun 2023
SRR074369.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074369.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8036740
Total Bases329.5 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAA3985254.958789260321971No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA2366082.944079315742453No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA1954452.431894026682461No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAA1827212.2735711246102275No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA1368181.7024066972429122No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA1300191.6178077180548331No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA1112781.3846161503296113No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA1026731.2775453728750712No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAA859851.0698989888935064No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAA773680.9626788971647707No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA747390.9299666282597173No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAA682970.8498097487289623No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA564560.7024738886663No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAA498610.6204132521395491No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA481990.5997332251634369No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAAAA459150.5713137416415114No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA451330.5615834281064213No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAAA382880.47641207753392545No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA369860.4602114787836859No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAA358340.44587730846089335No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAA345630.43006243825232615No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA339560.42250962454925756No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAA334150.41577803935426555No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAA328750.4090588970154565No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA312160.3884161986078933No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA311960.38816734148423365No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAA305140.3796813135674415No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAA292310.3637171290846786No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT282540.3515604585939075No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA259050.32233218942008823No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA230600.2869322635795111No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA220550.27442719311561653No Hit
ACGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA220510.2743774216908846No Hit
TGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAAAAA220440.27429032169760376No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCAAAAAAAAAAAAAAA207240.25786575153607055No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCAAAAAAAAAAAAAA194880.24248638129390773No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAA193950.2413291956688906No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAA193890.24125453853179277No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA193160.24034621003043524No Hit
TTACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAA181930.22637288253694907No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT177840.22128375435811037Illumina PCR Primer Index 1 (95% over 21bp)
TGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAAA176940.22016389730164218No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAA168640.20983632666976912No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT164610.20482185562802827No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAA162910.20270657007692172No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA158360.197045070513666No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA157710.1962362848617723No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA157310.19573857061445313No Hit
TGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA154900.19273984227435503No Hit
TGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAAA152510.18976599964662288No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCAAAAAAA152280.18947981395441435No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAA152080.18923095683075475No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA150960.18783735693826104No Hit
CTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAA149740.1863193284839375No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAA145170.1806329432083158No Hit
TTAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAA142860.1777586434300475No Hit
AGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA141320.17584244357786863No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT139840.17400090086278763No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA135130.16814031560060422No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAA133690.16634854431025514No Hit
CTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAA133540.16616190146751045No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAA131670.16383508736129326No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAA129690.1613714018370633No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA129060.16058750189753557No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAA127920.15916901629267588No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAAAAAAAAAAA126320.15717815930339915No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA126240.1570786164539353No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA125460.1561080736716629No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA124630.15507531660847557No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAA124320.15468958806680322No Hit
TAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAAAA120680.1501603884161986No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAA119920.14921473134629215No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAA118710.1477091457481516No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAA114690.1427071175625938No Hit
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT112080.13945953209883608Illumina PCR Primer Index 2 (95% over 21bp)
TGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAA111810.13912357498189565No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT111580.1388373892896871Illumina PCR Primer Index 2 (95% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCAAAAAAAAAAAAAAA111400.13861341787839349No Hit
TAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAA110940.13804104649397642No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAA109290.13598797522378478No Hit
AAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG108070.13446994676946125Illumina PCR Primer Index 2 (95% over 22bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAA104160.12960479000191621No Hit
TATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAAAAAAA103680.12900753290513317No Hit
TTACCCGTCGCCGCGCTCTCTCTCTCCCAAAAAAAAAAAAA103310.12854714722636293No Hit
AAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTGA103070.1282485186779714Illumina Paired End PCR Primer 2 (95% over 22bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAAAAAAAAAAAA101600.1264194188190734No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA101340.12609590455831593No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA99850.12424191898705197No Hit
CCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA99320.12358244760935404No Hit
ACAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA99230.12347046190370724No Hit
TGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAA98690.12279854766982631No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT98190.12217640486067732No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAA97260.12101921923566024No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAAA97210.12095700495474533No Hit
TTTGTCTTCATCATGTCTGGTCGTGGCAAAAAAAAAAAAAA96930.12060860498162189No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAAAAAAAA96170.11966294791171544No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA95160.1184062194372345No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAA93420.11624116246139603No Hit
ACAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC93420.11624116246139603No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAAA92300.11484756256890231No Hit
ACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAAAAAA91310.11361571980678734No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAA90990.11321754840893199No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAA89160.1109405057274467No Hit
GCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA87900.10937270584839126No Hit
CCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAAAAA87570.10896209159435293No Hit
AGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA87270.10858880590886354No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT86410.10751872027712729No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCACAAAAAAAAA85990.10699612031744214No Hit
TAGACTCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAA84570.10522923473945903No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA83060.1033503634558291No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAAAAAAAAAA82630.10281532063996099No Hit
TATCCGGTAAAGCGAATGATTAGAGGCAAAAAAAAAAAAAA81510.10142172074746726No Hit
CTAAACCATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA81390.1012724064732715Illumina PCR Primer Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)